Improving the biotechnology of symbiotic bacterial concentrate

I. V. Boyarineva, I. S. Khamagaeva
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Abstract

In the field of food technology, it is currently important to obtain bacterial concentrates with high yield and viability of microbial cells. Purposeful regulation of the technological parameters of the production of bacterial preparations has a significant impact on their quality and on the quality of finished food products. The results of research on improving the technology of liquid bacterial concentrate based on symbiotic starter culture for bakery production are presented. This bacterial concentrate consists of a consortium of microorganisms characteristic of the fermentation microflora of rye starter cultures. The optimal dose of inoculate application was determined in order to increase the biomass. A feature of the technology is a new approach to inoculate preparation and biomass production. In the production of symbiotic concentrate, it is important to optimize the composition of the nutrient medium, to create conditions for the growth of mesophilic lactobacilli and yeast that do not ferment lactose, characteristic of the fermentation microflora used in bakery production. To obtain biomass, a simple and cheap nutrient medium based on curd whey is used; the inoculum is a natural symbiotic starter culture for rye bread; due to the balanced composition of the medium, the process of biomass accumulation is intensified; biomass is characterized by a high titer of viable cells. A high survival rate of cells under infrared drying conditions has been established. The starter concentrate has high enzymatic activity, good lifting power, has a high titer of mesophilic lactobacilli and yeast that do not ferment lactose.
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共生菌浓缩液生物技术的改进
在食品技术领域,目前重要的是获得高产量和微生物细胞活力的细菌浓缩物。对细菌制剂生产的工艺参数进行有目的的调控,对其质量和成品食品质量都有重大影响。介绍了以共生发酵剂为基础,改进面包生产用浓缩液体细菌工艺的研究结果。这种细菌浓缩物由一群具有黑麦发酵剂发酵微生物群特征的微生物组成。为了提高生物量,确定了最佳接种剂量。该技术的一个特点是为接种制备和生物质生产提供了新的途径。在共生浓缩物的生产中,重要的是优化营养培养基的组成,为不发酵乳糖的嗜酸性乳酸杆菌和酵母的生长创造条件,这是烘焙生产中使用的发酵微生物群的特点。为了获得生物质,使用了一种简单廉价的以凝乳乳清为基础的营养培养基;接种物是一种天然的黑麦面包共生发酵剂;由于培养基成分平衡,生物质积累过程加剧;生物量的特点是高滴度的活细胞。在红外干燥条件下,细胞具有较高的存活率。该发酵剂浓缩物酶活性高,提升力好,具有高滴度的不发酵乳糖的嗜酸性乳酸杆菌和酵母。
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发文量
70
审稿时长
8 weeks
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