Influence of cytostatics on relative gene expression in redox status, apoptosis and migration colorectal carcinoma model system

Kragujevac Journal of Science Pub Date : 2023-01-01 DOI:10.5937/kgjsci2345159p

Jelena Pavić, Marko Živanović, Katarina Virijević, Irena Tanasković, Vesna Stanković, Nebojša Marić, Danijela Cvetković, Nenad Filipović

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Abstract

Colorectal cancer is one of the leading causes of mortality worldwide. It is the most common malignancy and there is a need for new approaches in therapies. Surgery, radiation and chemotherapy are the key components of colon cancer treatment. Besides common chemotherapy, alternative therapies are being studied to increase treatment effectiveness and reduce side effects. In this article, colorectal carcinoma cells were treated with chemotherapeutics and relative gene expression was investigated for the genes coding cytoskeleton proteins: CDH1, CTNNB1 and CDH2; for redox status genes: GPX1, GPX2, GPX3, GPX4, TXNRD1, GSTP1, NFE2L2, NFKB1, HIF1A; and for apoptosis genes: CASP3, CASP8, CASP9, FAS, BCL-2 and BAX. The results of our research showed that some concentrations of chemotherapeutics increased the expression of certain genes. Molecular alterations that lead to colorectal cancer can determine appropriate and effective treatment - chemotherapeutics, as well as the design of direct therapeutic targets.

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细胞抑制剂对大肠癌模型系统氧化还原状态、凋亡和迁移相关基因表达的影响

结直肠癌是世界范围内导致死亡的主要原因之一。它是最常见的恶性肿瘤，需要新的治疗方法。手术、放疗和化疗是结肠癌治疗的关键组成部分。除了普通的化疗，人们还在研究替代疗法，以提高治疗效果并减少副作用。本文用化疗药物治疗结直肠癌细胞，研究了细胞骨架蛋白编码基因CDH1、CTNNB1和CDH2的相关基因表达;氧化还原状态基因:GPX1、GPX2、GPX3、GPX4、TXNRD1、GSTP1、NFE2L2、NFKB1、HIF1A;凋亡基因:CASP3、CASP8、CASP9、FAS、BCL-2和BAX。我们的研究结果表明，某些浓度的化疗药物增加了某些基因的表达。导致结直肠癌的分子改变可以决定适当和有效的治疗-化疗，以及直接治疗靶点的设计。

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Kragujevac Journal of Science

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4 weeks