Determination of Peach Gum in Ancient Chinese Paintings by Biotin–Streptavidin-Enzyme-Linked Immunosorbent Assay

IF 0.8 4区化学 0 ARCHAEOLOGY Studies in Conservation Pub Date : 2023-02-09 DOI:10.1080/00393630.2023.2174798

Meng Wu, Bingjian Zhang, Qiong Zhang, Qian Cheng, Siwei Jiang, Li Li

引用次数: 0

Abstract

Peach gum was the most common and widely used exudate gum in ancient China. In this study, polyclonal antibodies to peach gum were prepared using the protein of peach gum as the antigen. The polyclonal antibodies were used to identify peach gum with high sensitivity and specificity by biotin–streptavidin-enzyme-linked immunosorbent assay (BA-ELISA). The binders used in the ancient paintings of the Forbidden City and the Dazu Rock Carvings were detected by enzyme-linked immunosorbent assay (ELISA). The results showed that the binders of the Dazu Rock Carvings were diverse, including collagen, ovalbumin, casein, peach gum, and lacquer, and that collagen and peach gum coexisted in most samples of the Forbidden City. This study demonstrates ELISA's high efficiency in analyzing binders in ancient paintings.

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生物素-链亲和素-酶联免疫吸附法测定古画中桃胶的含量

桃胶是中国古代最常见、应用最广泛的渗出胶。本研究以桃胶蛋白为抗原制备了针对桃胶的多克隆抗体。采用生物素-链亲和素-酶联免疫吸附试验(BA-ELISA)对桃胶进行了高灵敏度和特异性的鉴定。采用酶联免疫吸附法(ELISA)对紫禁城古画和大足石刻中使用的黏合剂进行了检测。结果表明，大足石刻的黏合剂种类繁多，有胶原蛋白、卵白蛋白、酪蛋白、桃胶、漆器等，大部分紫禁城石刻样品中胶原蛋白和桃胶共存。本研究验证了ELISA对古代绘画中黏合剂的高效分析。

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来源期刊

Studies in Conservation 化学-分析化学

CiteScore

1.80

自引率

12.50%

发文量

审稿时长

>12 weeks

期刊介绍： Studies in Conservation is the premier international peer-reviewed journal for the conservation of historic and artistic works. The intended readership includes the conservation professional in the broadest sense of the term: practising conservators of all types of object, conservation, heritage and museum scientists, collection or conservation managers, teachers and students of conservation, and academic researchers in the subject areas of arts, archaeology, the built heritage, materials history, art technological research and material culture. Studies in Conservation publishes original work on a range of subjects including, but not limited to, examination methods for works of art, new research in the analysis of artistic materials, mechanisms of deterioration, advances in conservation practice, novel methods of treatment, conservation issues in display and storage, preventive conservation, issues of collection care, conservation history and ethics, and the history of materials and technological processes. Scientific content is not necessary, and the editors encourage the submission of practical articles, review papers, position papers on best practice and the philosophy and ethics of collecting and preservation, to help maintain the traditional balance of the journal. Whatever the subject matter, accounts of routine procedures are not accepted, except where these lead to results that are sufficiently novel and/or significant to be of general interest.