Cyanide determination in postmortem blood samples using Headspace-Ion Mobility Spectrometry (HS-IMS)

IF 2.6 3区医学 Q2 CHEMISTRY, ANALYTICAL Forensic Chemistry Pub Date : 2023-10-27 DOI:10.1016/j.forc.2023.100539

Ali Moaddeli , Mehran Fereidooni , Marzieh Nabipour , Razieh Parchami , Mahmoud Tabrizchi

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Abstract

Interest in facile and sensitive methods for cyanide detection is related to the extreme cyanide toxicity and its importance in forensic toxicology. In this research a novel application of Ion Mobility Spectrometry (IMS) was demonstrated for rapid determination of cyanide poisoning in postmortem toxicology. In addition, a simple method for the sample preparation was applied based on the analyte (cyanide anion) transfer as HCN to the headspace and direct injection to IMS. The method showed a linear dynamic range of 50–2000 µg/L with R² > 0.99, a high sensitivity (LOD of 20.4 µg/L and LOQ of 68.1 µg/L). Good repeatability (intra- and inter-assays, CV < 15 %) and excellent extraction recovery (82 -94 %) were obtained. After validation, the method was applied in the analysis of postmortem human blood samples in forensic cases. In all samples, the cyanide was promptly quantified.

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利用顶空离子迁移谱法（HS-IMS）测定死后血液样本中的氰化物含量

人们之所以对简便灵敏的氰化物检测方法感兴趣，与氰化物的剧毒及其在法医毒理学中的重要性有关。本研究展示了离子迁移谱法（IMS）在尸检毒理学中快速测定氰化物中毒的新应用。此外，还采用了一种简单的样品制备方法，将分析物（氰化物阴离子）以 HCN 的形式转移到顶空，然后直接注入 IMS。该方法的线性动态范围为 50-2000 µg/L，R2 为 0.99，灵敏度高（LOD 为 20.4 µg/L，LOQ 为 68.1 µg/L）。重复性好（测定内和测定间，CV < 15 %），萃取回收率高（82 -94 %）。经过验证后，该方法被用于分析法医案件中的死后人体血液样本。所有样本中的氰化物都能被迅速定量。

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来源期刊

Forensic Chemistry CHEMISTRY, ANALYTICAL-

CiteScore

5.70

自引率

14.80%

发文量

审稿时长

46 days

期刊介绍： Forensic Chemistry publishes high quality manuscripts focusing on the theory, research and application of any chemical science to forensic analysis. The scope of the journal includes fundamental advancements that result in a better understanding of the evidentiary significance derived from the physical and chemical analysis of materials. The scope of Forensic Chemistry will also include the application and or development of any molecular and atomic spectrochemical technique, electrochemical techniques, sensors, surface characterization techniques, mass spectrometry, nuclear magnetic resonance, chemometrics and statistics, and separation sciences (e.g. chromatography) that provide insight into the forensic analysis of materials. Evidential topics of interest to the journal include, but are not limited to, fingerprint analysis, drug analysis, ignitable liquid residue analysis, explosives detection and analysis, the characterization and comparison of trace evidence (glass, fibers, paints and polymers, tapes, soils and other materials), ink and paper analysis, gunshot residue analysis, synthetic pathways for drugs, toxicology and the analysis and chemistry associated with the components of fingermarks. The journal is particularly interested in receiving manuscripts that report advances in the forensic interpretation of chemical evidence. Technology Readiness Level: When submitting an article to Forensic Chemistry, all authors will be asked to self-assign a Technology Readiness Level (TRL) to their article. The purpose of the TRL system is to help readers understand the level of maturity of an idea or method, to help track the evolution of readiness of a given technique or method, and to help filter published articles by the expected ease of implementation in an operation setting within a crime lab.