Emine E. Alieva , Muniver T. Gafarova , Eugene I. Bondarenko , Igor A. Dovgan , Irina I. Osiptchuk , Marina E. Eremeeva
{"title":"Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report","authors":"Emine E. Alieva , Muniver T. Gafarova , Eugene I. Bondarenko , Igor A. Dovgan , Irina I. Osiptchuk , Marina E. Eremeeva","doi":"10.1016/j.imj.2023.09.004","DOIUrl":null,"url":null,"abstract":"<div><p>Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 <em>Rickettsia</em> protein genes. The strain causing clinical illness was characterized as <em>Rickettsia conorii</em> subspecies <em>conorii</em> Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management<em>.</em></p></div>","PeriodicalId":100667,"journal":{"name":"Infectious Medicine","volume":"2 4","pages":"Pages 338-342"},"PeriodicalIF":0.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772431X23000552/pdfft?md5=5e6e114e348e4112265cef7e4de1e575&pid=1-s2.0-S2772431X23000552-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infectious Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772431X23000552","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes. The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.
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