Development of SRAP markers to evaluate the genetic variability in Brachypodium distachyon complex

IF 1.5 4区生物学 Q3 PLANT SCIENCES Botany Letters Pub Date : 2023-09-09 DOI:10.1080/23818107.2023.2222458

María Inés Dinolfo, Eliana Castañares, Mauro Martínez, Florencia Arroyo, Sebastián Stenglein

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Abstract

ABSTRACT Brachypodium distachyon has been accepted as a model grass species for genetics and molecular genomics in cereals since 2001. However, the genetic variability present in Brachypodium spp. continue being the aim of many research. Therefore, the aim of this work was to analyze the genetic variability present in different Brachypodium spp. from different countries and to find different open reading frames (ORFs) among them by using sequence-related amplified polymorphism (SRAP) markers. In our study, a total of 12 Brachypodium spp. accessions were selected from different countries. By using SRAP markers, the genetic variability was evaluated and some fragments were sequenced to obtain differential ORF information among genotypes. SRAP molecular markers revealed high variability among accessions being USA the accession that showed the most variability at the DNA level. The analysis of sequenced SRAP fragments from monomorphic and polymorphic fragments as well as those obtained from new primer combinations targeted coding regions into B. distachyon available genome. SRAP molecular marker showed not only to be an excellent tool for Brachypodium variability studies but also to provide additional information about differential amplification of open reading frames.

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利用SRAP标记评价短柄草复合体遗传变异性

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来源期刊

Botany Letters Agricultural and Biological Sciences-Plant Science

CiteScore

3.10

自引率

6.70%

发文量

期刊介绍： Botany Letters is an international scientific journal, published by the French Botanical Society (Société botanique de France) in partnership with Taylor & Francis. Botany Letters replaces Acta Botanica Gallica, which was created in 1993, building on over a century of renowned publications by the Société botanique de France.