Characterization of a cDNA clone for barley leaf glutamine synthetase.

S Baima, A Haegi, P Strøman, G Casadoro
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引用次数: 12

Abstract

A barley cDNA clone (1182 bp) encoding chloroplastic glutamine synthetase was isolated with a heterologous cDNA probe of the gene specifying the enzyme from alfalfa. The clone, named pGS8, was found in a lambda gtII cDNA library prepared from dark grown barley leaves even though the chloroplastic glutamine synthetase is absent from such leaves. In agreement therewith the clone hybridized in Northern blot analyses with a 1.7 kb mRNA species present the in poly A+ mRNA fraction of both dark grown and greened primary leaves of barley. The nucleotide sequence of the barley clone reveals 75% identity to the Phaseolus vulgaris and Pisum sativum clones encoding chloroplastic glutamine synthetase, while only 69% identity is observed in comparisons with the clones specifying the cytosolic isozymes. At the amino acid level 85% identity is found between the deduced barley glutamine synthetase sequence and that of the corresponding chloroplastic isoenzymes from bean and pea. The chloroplastic glutamine synthetases contain cysteins in the putative ATP and and substrate binding sites. In the cytosolic forms these positions are occupied by alanine residues.

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大麦叶片谷氨酰胺合成酶cDNA克隆的鉴定。
利用苜蓿中叶绿体谷氨酰胺合成酶基因的cDNA探针,分离得到了一个编码叶绿体谷氨酰胺合成酶的大麦cDNA克隆(1182 bp)。这个克隆体被命名为pGS8,它是在一个从深色大麦叶片中制备的lambda gtII cDNA文库中发现的,尽管这种叶片中没有叶绿体谷氨酰胺合成酶。与此一致的是,克隆杂交的Northern blot分析显示,大麦暗生叶和绿生叶中均含有1.7 kb的多聚a + mRNA片段。大麦无性系的核苷酸序列与编码叶绿体谷氨酰胺合成酶的菜豆无性系和豌豆无性系的同源性为75%,而与胞质同工酶无性系的同源性仅为69%。在氨基酸水平上,推导出的大麦谷氨酰胺合成酶序列与大豆和豌豆中相应的叶绿体同工酶序列有85%的一致性。叶绿体谷氨酰胺合成酶在假定的ATP和底物结合位点含有半胱氨酸。在细胞质形式中,这些位置被丙氨酸残基占据。
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