A new technique for staining catecholic residues in biological samples.

R Kalyani, K Nellaiappan
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引用次数: 1

Abstract

This technique for localizing catecholic residues in biological samples is based on the condensation of Besthorn's hydrazone (3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) with quinone residues obtained by the oxidation of catechols in the presence of ammonia. The product is a dark pink MBTH-quinone compound. This method is very sensitive and positive to catechol even at the 0.05 microgram level and the final product is chemically stable.

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生物样品中儿茶酚残留染色新技术。
这种定位生物样品中儿茶酚残基的技术是基于贝索恩的腙(3-甲基-2-苯并噻唑啉酮肼盐酸盐(MBTH))与儿茶酚在氨存在下氧化得到的醌残基的缩合。该产品是一种深粉红色的四氢甲酚醌化合物。该方法对儿茶酚在0.05微克水平下也具有很高的灵敏度和阳性反应,且最终产物化学性质稳定。
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