Screening of Different Extraction Methods for Quantitative and Qualitative Analysis of Antioxidants and Assessment of Free Radical Scavenging Activity in Morinda citrifolia L.

Abstract

The interest of scientists and medical professionals in herbal medicinal products containing phytochemicals derived from plants is growing daily due to the shift of consumers’ preferences from convenience to environmental sustainability. One plant utilized in many medicines is Morinda citrifolia L., a tree commonly used in folk medicine, health supplements and immune-boosting products. In this study, Bioactive compounds from M . citrifolia were extracted using conventional methods and modern methods (Ultrasound-Assisted, Microwave Assisted, Water bath Assisted, Shaker Assisted, Soxhlet Assisted and Magnetic Stirrer Assisted Extraction). This study screens a variety of extraction methods used to extract active compounds: total phenolic content and antioxidant properties through DPPH, ABTS and FRAP from different parts of M. citrifolia . The results indicate that the total phenolic content and FRAP activity were highest in all parts of the plant in Soxhlet Assisted extraction. In contrast, Microwave Assisted extraction showed high DPPH and ABTS activity. This study offered valuable details on the effective phytochemical extraction processes from various parts of Morinda citrifolia L.