Development of the Experimental Method to Evaluate Sodium Ion Exclusion in the Mature Root Zone of <i>Arabidopsis thaliana</i>

Tomoki Nagata, Mio Nagoya, Takaaki Ogura, Yuko Kurita, Natsuko I. Kobayashi, Keitaro Tanoi
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Abstract

Sodium (Na) exclusion is vital for plants to cope with salt stress, and the plasma membrane Na+/H+ antiporter, SALT OVERLY SENSITIVE 1 (SOS1), has been known to play a central role in this process although the function of this molecule is not completely understood. In this study, we performed 22Na+ radiotracer experiments to investigate the function of SOS1 in the mature root zone. Arabidopsis thaliana seedlings were placed on the gel medium, which had air gaps to separate the medium at the mature root zone from that at the root apex zone. Then, 22Na+ was added to the root apex zone for 4 h, and the radioactivity in the mature root zone with 5 mm in length and in the mature root zone gel was measured. As a result, we found that knock-out of SOS1 caused 22Na+ accumulation in the mature root zone and reduction of 22Na+ exclusion to the gel, demonstrating that Na+ delivered via the xylem is excluded to the rhizosphere by SOS1 in the mature root zone.
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拟南芥成熟根区钠离子排斥试验方法的建立</i>
钠(Na)排斥对植物应对盐胁迫至关重要,质膜Na+/H+反转运蛋白salt oversensitive 1 (SOS1)在这一过程中起着核心作用,尽管该分子的功能尚不完全清楚。在本研究中,我们通过22Na+放射性示踪剂实验来研究SOS1在成熟根区的功能。拟南芥幼苗置于凝胶培养基中,凝胶培养基中存在气隙,将成熟根区和根尖区培养基分开。然后在根尖区添加22Na+ 4 h,测定长5mm的成熟根区和成熟根区凝胶的放射性。结果发现,敲除SOS1导致22Na+在成熟根区积累,减少了22Na+对凝胶的排异,表明成熟根区通过木质部输送的Na+被SOS1排除到根际。
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