A method for preparing and staining histological sections containing titanium implants for light microscopy.

K Gotfredsen, E Budtz-Jörgensen, L N Jensen
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引用次数: 79

Abstract

An improved method for preparing and staining ground tissue-implant sections for light microscopy is presented. Undecalcified tissue blocks with titanium implants were dehydrated in an ascending series of ethanol and stained in toto with basic fuchsin. Specimens were infiltrated and embedded in methyl methacrylate and sections were prepared using a cutting-grinding-system. The polished surface was counterstained with light green or anilin blue. Light polymerizing resin was used as slide mounting medium and for mounting the coverglass. The sections obtained were 10-15 microns thick with tissue architecture which clearly differentiated structures at the tissue-implant interface. The method was very useful for computer assisted morphometric analysis.

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一种制备和染色光学显微镜用含钛植入物的组织学切片的方法。
提出了一种光镜下制备和染色地面组织植入切片的改进方法。带钛植入物的未钙化组织块在递增系列乙醇中脱水,并用碱性品红染色。将标本浸润并包埋在甲基丙烯酸甲酯中,并用切割-研磨系统制备切片。抛光后的表面涂上了淡绿色或动物蓝。采用轻聚合树脂作为载玻片的贴装介质和盖板玻璃的贴装。获得的切片厚度为10-15微米,组织结构清晰,组织-植入物界面处结构分化明显。该方法对计算机辅助形态计量学分析非常有用。
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