Hoechst 33342 staining coupled with conventional histological technique.

W Sawicki, S Moskalewski
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引用次数: 13

Abstract

Hoechst 33342 was injected either intravenously or intraperitoneally into mice which were killed 1 or 24 hr or 7, 14 or 28 days later. Various organs were fixed and paraffin embedded. Visual inspection showed that independently of the route of dye administration or survival time, distinct fluorescence of nuclei was observed in organs other than cerebral cortex. Even formic acid decalcification of bone failed to abolish the fluorescence of osteocytes. In vivo staining with Hoechst 33342 is proposed as an alternative for staining after sectioning. Cells from spleens of Hoechst 33342-injected mice or stained in vitro were injected intramuscularly into mice. Hoechst 33342-stained splenocytes could be found in deparaffinized sections at the site of injection 24 hr later.

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Hoechst 33342染色结合常规组织学技术。
将Hoechst 33342分别静脉或腹腔注射到小鼠体内,分别于1、24小时或7、14、28天后死亡。固定各脏器,石蜡包埋。肉眼观察显示,与给药途径或存活时间无关,除大脑皮层外,其他器官均可见明显的细胞核荧光。即使甲酸脱钙也不能消除骨细胞的荧光。建议用Hoechst 33342进行体内染色,作为切片后染色的替代方法。取Hoechst 33342小鼠脾细胞肌内注射或体外染色。24小时后,在注射部位脱胶切片上可见Hoechst 33342染色的脾细胞。
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