Pistacia Türlerinin rbcL Kloroplast Gen Bölgesi ile Filogenetik Analizi

Abstract

The phylogeny of five species in the genus Pistacia was assessed using the plastid rbcL gene region. Genomic DNA was isolated from leaf tissue and PCR analysis with primer of rbcL gene region and DNA sequencing were performed. Using obtained sequence data, phylogenetic and PCoA (Principle Component Analysis) analyses were performed for reveal the phylogenetic relationships among Pistacia species. According to the result of phylogenetic analysis, closer grouping in clades was observed between P. vera; P. khinjuk and P. atlantica; between P. palaestina and P. terebinthus. Result of PCoA analysis supported the result of phylogenetic analysis. As a result, rbcL gene region was found powerful at species-based grouping. Revealed sequence information of chloroplast gene region is reliable to elaborate a molecular database to conduct breeding programs on local pistachio gene pool.