The optimisation and standardisation of Indonesian house dust mites allergenic extract as a desensitising agent

Abstract

Background: The different sequences of the allergen protease can induce different immunogenicity for the development of immunotherapy. Dermatophagoides pteronyssinus that has Der p1 as major protein will be developed as local allergen to be immunotherapy for treating rhinitis allergy. Objective: This study aims to optimize and standardize the local Indonesian House Dust Mites Allergenic Extract from the harvesting into the extraction process. Method: The harvesting media used is corn flour placed in certain conditions to produce the mites. After harvesting the flour in different weeks, the flour was extracted and the lipid content and Der p1 assay were measured. Result: The result of the study is that the 3 weeks-harvesting time came higher for those parameters than the other harvesting time (p < 0.05). Conclusion: Thus, it was concluded that the standardized harvesting and extraction process had been developed to produce the standardised Indonesian House Dust Mites Allergenic Extract.