[Computer analysis of single channel current record by extracellular patch clamp].

Abstract

Neher and Sakmann had provided the presence of the channel protein by the direct measurement of single channel current from excitable membrane in 1976. The advantage of this method "patch clamp" is to isolate a small part of membrane and pick up electrical excitability. In order to record single channel current the seal resistance between patch electrode and cell membrane must be at least more than gigaohm. To improve this recording condition enzyme treated cell or culture cell is necessary since fibroblast which attaches to a target cell disturbs gigaseal formation. In this study we use the computer to improve the single channel current recorded by low seal resistance.