Callus induction studies in various explants in different genotypes of sesame (Sesamum indicum L.)

Abstract

The goal of this study was to develop a suitable protocol for callus induction in sesame, Sesamum indicumwhich can be further used for regeneration studies. The experiments were carried out using three explants viz.,cotyledonary leaf, hypocotyls and primary leaf at different hormonal combinations and concentrations to studycallus induction. There was significant difference in the response of explants to different hormone treatmentsalthough there is no genotypic difference with regard to explants. Results showed that callus induction wassignificantly influenced by explants type. Hypocotyls responded well for callus induction in most of the genotypes.The callus induction frequency was significantly reduced (36.5%) when 0.2 mg BA was used along with 0.2 mg/lNAA. Replacing this hormonal combination with 2.0mg/l BA and 0.2 mg/l NAA greatly improved the callusinduction frequency (51.4%).