Character association and molecular characterization of winged bean (Psophocarpus tetragonolobus L.)

Abstract

In order to select high yielding winged bean genotypes, genetic variations between the winged bean genotypes were studied to determine the genetic link between seed yield and yield contributing factors. A study was conducted during kharif season 2021-2022 on morphological and molecular characterization of 20-winged bean genotypes (Psophocarpus tetragonolobus) for 10 quantitative traits along with 16 ISSR markers were used to evaluate promising and suitable genotypes for cultivation and research. According to the findings of the study, it was observed that the genotype ‘IC 112416’ exhibited the greatest pod length, measuring 17.8 cm, as well as the highest number of seeds per pod with an average value of 11.8 seed/pod. We found similar results from the study of genetic components and association analysis as PCV (24.4), GCV (23.25), heritability (90.15%) and genetic advance (45.48%). Here we have standardized a DNA extraction protocol for isolating a quantified DNA using CTAB and PVP extraction method, which is further used in different molecular studies. From the molecular analysis it was found that 12 markers were polymorphic in nature with high allelic frequency. The PIC (Polymorphic information content) of each marker is recorded and cluster analysis of molecular data is done using Jaccard’s similarity coefficient with the help of dendrogram.