Immunomodulation of polymorphonuclear leukocytes by D53 Immucytal and its constitutive fractions.

Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01

F Minonzio, A M Ongari, E Venegoni, V Carbonelli, L Licciardello, F Capsoni

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Abstract

D53 (Immucytal) is a compositive vaccine made of immunogenic ribosomes extracted from 4 bacterial species (Klebsiella pneumoniae, Haemophilus influenzae, Streptococcus pyogenes and Streptococcus pneumoniae) associated with a membrane proteoglycan from a non-encapsulated strain of Klebsiella pneumoniae (MPG-Kp). In this work we have studied the effect of the compound on human polymorphonuclear leukocyte (PMN) function "in vitro". We have demonstrated that D53 was able to significantly increase Fc- receptor dependent phagocytosis without modify the C3-receptor dependent activity. Furthermore D53 enhanced the oxidative metabolism (evaluated by chemiluminescence) both using cells in resting conditions or after stimulation with phagocytable or soluble stimuli. On the contrary D53 caused a dose-dependent inhibition of PMN migration toward different chemoattractants. Using the two constitutive fractions of the compound (ribosomes and proteoglycans) we have observed that the MPG-Kp component was mainly responsible for the modulating activity of the drug on human PMNs.

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D53免疫细胞及其组成部分对多形核白细胞的免疫调节作用。

D53 (Immucytal)是一种复合疫苗，由从4种细菌(肺炎克雷伯菌、流感嗜血杆菌、化脓性链球菌和肺炎链球菌)中提取的免疫原性核糖体与从肺炎克雷伯菌(MPG-Kp)非包膜菌株中提取的膜蛋白聚糖结合而成。在这项工作中，我们研究了化合物在体外对人多形核白细胞(PMN)功能的影响。我们已经证明，D53能够显著增加Fc受体依赖的吞噬作用，而不改变c3受体依赖的活性。此外，D53增强了细胞的氧化代谢(通过化学发光来评估)，无论是在静止状态下，还是在吞噬性或可溶性刺激刺激后。相反，D53对PMN向不同化学引诱剂的迁移具有剂量依赖性的抑制作用。使用化合物的两个组成部分(核糖体和蛋白多糖)，我们观察到MPG-Kp成分主要负责药物对人pmn的调节活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Bollettino dell'Istituto sieroterapico milanese

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