Synthetic peptides of IL-1Ra and HSP70 have anti-inflammatory activity on human primary monocytes and macrophages: Potential treatments for inflammatory diseases

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2023-11-17 DOI:10.1016/j.nano.2023.102719
Alba Pensado-López PhD , Aldo Ummarino PhD Student , Sophia Khan PhD , Anna Guildford PhD , Iain U. Allan PhD , Matteo Santin PhD , Nathalie Chevallier PhD , Elina Varaillon MSc, PhD , Elizaveta Kon MD , Paola Allavena MD , Fernando Torres Andón PhD
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Abstract

Chronic inflammatory diseases are increasing in developed societies, thus new anti-inflammatory approaches are needed in the clinic. Synthetic peptides complexes can be designed to mimic the activity of anti-inflammatory mediators, in order to alleviate inflammation. Here, we evaluated the anti-inflammatory efficacy of tethered peptides mimicking the interleukin-1 receptor antagonist (IL-1Ra) and the heat-shock protein 70 (HSP70). We tested their biocompatibility and anti-inflammatory activity in vitro in primary human monocytes and differentiated macrophages activated with two different stimuli: the TLR agonists (LPS + IFN-γ) or Pam3CSK4. Our results demonstrate that IL-1Ra and HSP70 synthetic peptides present a satisfactory biocompatible profile and significantly inhibit the secretion of several pro-inflammatory cytokines (IL-6, IL-8, IL-1β and TNFα). We further confirmed their anti-inflammatory activity when peptides were coated on a biocompatible material commonly employed in surgical implants. Overall, our findings support the potential use of IL-1Ra and HSP70 synthetic peptides for the treatment of inflammatory conditions.

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IL-1Ra和HSP70合成肽对人原代单核细胞和巨噬细胞具有抗炎活性:炎症性疾病的潜在治疗方法
慢性炎症性疾病在发达社会越来越多,因此临床需要新的抗炎方法。合成肽复合物可以被设计成模拟抗炎介质的活性,以减轻炎症。在这里,我们评估了模拟白细胞介素-1受体拮抗剂(IL-1Ra)和热休克蛋白70 (HSP70)的栓系肽的抗炎功效。在TLR激动剂(LPS + IFN-γ)和Pam3CSK4两种不同刺激下,我们在体外测试了它们在原代人单核细胞和分化巨噬细胞中的生物相容性和抗炎活性。我们的研究结果表明,IL-1Ra和HSP70合成肽具有令人满意的生物相容性,并显著抑制几种促炎细胞因子(IL-6、IL-8、IL-1β和TNFα)的分泌。我们进一步证实了它们的抗炎活性,当肽包被在生物相容性材料通常用于外科植入物。总的来说,我们的研究结果支持IL-1Ra和HSP70合成肽治疗炎症的潜在应用。
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来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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