{"title":"Chemical cross-linking analysis of Ca2+-ATPase from rabbit skeletal muscle","authors":"Brendan E. Murray, Kay Ohlendieck","doi":"10.1016/S0307-4412(98)00268-4","DOIUrl":null,"url":null,"abstract":"<div><p>A student laboratory experiment is described dealing with chemical cross-linking analysis of Ca<sup>2+-</sup>ATPase from rabbit muscle. The experiment introduces students to a wide range of techniques including subcellular fractionation, chemical cross-linking, polyacrylamide gel electrophoresis, blotting and immunochemical detection of proteins.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 1","pages":"Pages 41-46"},"PeriodicalIF":0.0000,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(98)00268-4","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical education","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0307441298002684","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A student laboratory experiment is described dealing with chemical cross-linking analysis of Ca2+-ATPase from rabbit muscle. The experiment introduces students to a wide range of techniques including subcellular fractionation, chemical cross-linking, polyacrylamide gel electrophoresis, blotting and immunochemical detection of proteins.
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