Micronuclei frequency in circulating erythrocytes from rainbow trout (Oncorhynchus mykiss) subjected to radiation, an image analysis and flow cytometric study
N. Schultz , L. Norrgren, J. Grawé, A. Johannisson, Ö. Medhage
{"title":"Micronuclei frequency in circulating erythrocytes from rainbow trout (Oncorhynchus mykiss) subjected to radiation, an image analysis and flow cytometric study","authors":"N. Schultz , L. Norrgren, J. Grawé, A. Johannisson, Ö. Medhage","doi":"10.1016/0742-8413(93)90196-R","DOIUrl":null,"url":null,"abstract":"<div><p>1. Rainbow trout (<em>Oncorhynchus mykiss</em>) were exposed to a single X-ray dose of 4Gy.</p><p>2. The frequency of micronuclei in the peripheral erythrocytes was investigated at regular intervals up to 58 days after the exposure.</p><p>3. A flow cytometric method and a semi-automatic image analysis method were used to estimate the micronuclei frequency.</p><p>4. The results show that both methods can detect an increased frequency of micronuclei in peripheral erythrocytes from exposed fish.</p><p>5. However, the semi-automatic image analysis method was the most stable and sensitive.</p></div>","PeriodicalId":72650,"journal":{"name":"Comparative biochemistry and physiology. C: Comparative pharmacology","volume":"105 2","pages":"Pages 207-211"},"PeriodicalIF":0.0000,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0742-8413(93)90196-R","citationCount":"35","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative biochemistry and physiology. C: Comparative pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/074284139390196R","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 35
Abstract
1. Rainbow trout (Oncorhynchus mykiss) were exposed to a single X-ray dose of 4Gy.
2. The frequency of micronuclei in the peripheral erythrocytes was investigated at regular intervals up to 58 days after the exposure.
3. A flow cytometric method and a semi-automatic image analysis method were used to estimate the micronuclei frequency.
4. The results show that both methods can detect an increased frequency of micronuclei in peripheral erythrocytes from exposed fish.
5. However, the semi-automatic image analysis method was the most stable and sensitive.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
