The use of bromodeoxyuridine-immunohistochemistry to identify transplanted fetal brain tissue.

D B Brown, B B Stanfield
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引用次数: 7

Abstract

The immunohistochemical detection of the thymidine analog, 5-bromo-2'-deoxyuridine (BrdU) is shown to be a useful and reliable method to positively identify fetal brain transplants in standard histological preparations. This technique offers several advantages over the [3H]thymidine autoradiographic method, including being much more rapid and avoiding the use of radionuclides.

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应用溴脱氧尿嘧啶免疫组织化学鉴别移植胎儿脑组织。
胸腺嘧啶类似物5-溴-2'-脱氧尿嘧啶(BrdU)的免疫组织化学检测被证明是一种有用和可靠的方法,可以在标准组织学准备中阳性识别胎儿脑移植。与[3H]胸苷放射自显影法相比,该技术有几个优点,包括更快,避免使用放射性核素。
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