Expression and regulation of interleukin-1 mRNA and interleukin-1 receptors in human B-cell lines.

Abstract

Long-term human lymphoid B-cell lines have been described to produce a number of growth factors, including interleukin-1 (IL-1) which may be of importance in the autocrine growth regulation of these cells and may participate in their antigen presenting function. In this report, we have analyzed the production of IL-1 by 12 established B-cell lines at the level of mRNA expression. Among the 5 cell lines containing an IL-1 message, three expressed exclusively IL-1 alpha, one contained traces of IL-1 beta, and only one line contained both. The steady-state level of IL-1 alpha mRNA in these cells could be drastically increased by a short culture of the cells with the protein synthesis inhibitor cycloheximide or with PMA. PMA, however, induced a transient increase in mRNA which could be stabilized by Ca2+ ionophore. Furthermore, only constitutively produced IL-1 mRNA are increased by these compounds which do not induce de novo transcription of silent IL-1 genes in these lines. These data provide the basis for further investigations on the regulation of IL-1 mRNA expression in human B cells. In addition, we studied expression of IL-1 receptors in these lines and observed that only cells which produce IL-1, displayed IL-1 receptors at their surface, as detected by radiolabeled IL-1 binding experiments. These data strongly suggest an autocrine role for IL-1 in these lines. IL-1 mRNA and IL-1 receptors were reciprocally regulated by PMA, which increased IL-1 mRNA and lowered the number of receptors.(ABSTRACT TRUNCATED AT 250 WORDS)