Effect of a hyperlipidic diet on lipid composition, fluidity, and (Na+-K+)ATPase activity of rat erythrocyte membranes.

Membrane biochemistry Pub Date : 1989-01-01 DOI:10.3109/09687688909025822

A Bordoni, P L Biagi, G Parenti Castelli, S Hrelia, C A Rossi, G Lercker, J C Izpisua, T Barber, J Cabo, G Lenaz

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引用次数: 11

Abstract

Feeding rats a hyperlipidic diet in which animals were offered daily a variety of high-energy food resulted in a significant increase of serum free fatty acids and a decrease of phospholipids with respect to controls. On the contrary, there were no significant differences in erythrocyte membrane total lipid composition between the two groups. Erythrocyte membranes showed a significant decrease in saturated fatty acid content and a significant increase in (n-6) polyunsaturated fatty acid content; (n-3) polyunsaturated fatty acids significantly decreased. Membrane fluidity, investigated by fluorescence polarization of diphenylhexatriene, significantly increased in the erythrocyte membranes of the experimental group. These results seem compatible with the decreased saturated/unsaturated fatty acid ratio. A significant decrease of (Na+-K+)ATPase activity occurred in erythrocyte membranes of the experimental group rats with respect to the controls.

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高脂饮食对大鼠红细胞膜脂质组成、流动性和(Na+-K+) atp酶活性的影响

给大鼠喂高脂饮食，每天给动物提供各种高能量食物，结果与对照组相比，血清游离脂肪酸显著增加，磷脂显著减少。相反，两组间红细胞膜总脂质组成无显著差异。红细胞膜饱和脂肪酸含量显著降低，(n-6)多不饱和脂肪酸含量显著升高;(n-3)多不饱和脂肪酸显著减少。用二苯己三烯荧光偏振法观察细胞膜流动性，实验组红细胞膜流动性明显增加。这些结果似乎与饱和/不饱和脂肪酸比例的降低是一致的。与对照组相比，实验组大鼠红细胞膜(Na+-K+) atp酶活性明显降低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Membrane biochemistry

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