[Results and experiences with several methods for the immunodiagnosis of amebiasis].

Angewandte Parasitologie Pub Date : 1989-11-01
H Sauer, G Ockert, S Schubert, G Rothenberg, H E Stein, A Teichmann
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Abstract

Results and experiences of some immunodiagnostic methods for amebiasis. Experiences concerning the laboratory diagnosis of invasive amebiasis are described and discussed. Technics used include the Passive Hemagglutination Test with E. histolytica antigen (Behring FRG) and sheep erythrocytes as carrier and the Indirect Fluorescence Antibody Reaction (IFAR) with corpuscular antigen prepared from cultures of E. histolytica strain HK 9. Comparatively for a part infected hamster liver (Dr. Richle strain) has been used as antigen. We conclude that IFAR with HK 9 antigen is more sensitive than the other tests. It has been proved useful in the diagnosis of extraintestinal amebiasis (antibody titer greater than or equal to 1:160).

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几种阿米巴病免疫诊断方法的结果与体会。
几种阿米巴病免疫诊断方法的结果与体会。对侵袭性阿米巴病的实验室诊断经验进行了描述和讨论。采用溶组织芽孢杆菌抗原(Behring FRG)和绵羊红细胞为载体的被动血凝试验和用溶组织芽孢杆菌HK 9培养的红细胞抗原制备的间接荧光抗体反应(IFAR)。相对而言,部分感染的仓鼠肝脏(Dr. Richle株)已被用作抗原。结果表明,含hk9抗原的IFAR比其他检测方法更敏感。它已被证明对肠外阿米巴病的诊断有用(抗体滴度大于或等于1:160)。
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