1 alpha, 25-dihydroxyvitamin D3 enhances the up-regulation of interleukin-2 receptor (p55) by interleukin-2.

Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society Pub Date : 1989-09-01

S Tamori, T Uchiyama, H Uchino

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Abstract

The effect of 1 alpha, 25-dihydroxyvitamin D3 (1 alpha, 25(OH)2D3) on the expression of interleukin-2 (IL-2) receptor in activated T lymphocytes was examined. 1 alpha, 25(OH)2D3 enhanced the expression of IL-2 receptor (p55, Tac peptide) in phytohemagglutinin (PHA)-stimulated (3 days) human peripheral blood mononuclear cells (PBM) only in the presence of IL-2 without affecting the proliferation of the cells. This enhancement was dependent on the concentration of both IL-2 (0-1 U/ml) and 1 alpha, 25(OH)2D3(0-10(-7)M). The addition of interleukin-1 (IL-1, 0-100 U/ml), did not enhance the expression of IL-2 receptor in these cells in the presence of IL-2. Moreover, 1 alpha, 25(OH)2D3 had the same effect on two cell lines, Kit225 (an IL-2 dependent cell line established from a patient with T cell chronic lymphocytic leukemia) and YT (an IL-2 independent natural killer (NK)-like cell line from a patient with acute lymphocytic leukemia). Thus, 1 alpha, 25(OH)2D3 enhances the up-regulation of IL-2 receptor (p55) by IL-2 not only in activated T cells but also in the NK-like cell line.

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1 α， 25-二羟基维生素D3增强白介素-2对白细胞介素-2受体(p55)的上调。

研究了1 α， 25-二羟基维生素D3 (1 α， 25(OH)2D3)对活化T淋巴细胞白细胞介素-2 (IL-2)受体表达的影响。1 α， 25(OH)2D3仅在IL-2存在的情况下增强植物血凝素(PHA)刺激(3天)人外周血单核细胞(PBM)中IL-2受体(p55, Tac肽)的表达，而不影响细胞的增殖。这种增强依赖于IL-2 (0-1 U/ml)和1 α， 25(OH)2D3(0-10(-7)M)的浓度。白细胞介素-1 (IL-1, 0 ~ 100 U/ml)的加入对IL-2存在的细胞中IL-2受体的表达没有增强作用。此外，1 α， 25(OH)2D3对两种细胞系Kit225(来自T细胞慢性淋巴细胞白血病患者的IL-2依赖性细胞系)和YT(来自急性淋巴细胞白血病患者的IL-2非依赖性自然杀伤(NK)样细胞系)具有相同的作用。因此，1 α， 25(OH)2D3增强IL-2对IL-2受体(p55)的上调，不仅在活化的T细胞中，而且在nk样细胞系中也是如此。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society

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