[Yersinia enterocolitica--its multiplication on nutrient media containing rhamnose, raffinose and sodium succinate].

Abstract

The kinetics of growth in vitro of strains Yersinia enterocolitica, serovars 0:3, 0:5, 0:8 and 0:9, on culture media containing rhamnose, raffinose and sodium succinate and at different temperatures of cultivation (4 degrees, 25 degrees and 37 degrees C) was characterized. It was established, that all the Yersinia enterocolitica serovars tested, cultivated at 25 degrees C, had the highest growth rate. A significant activation of growth at this temperature was observed when L-rhamnose and especially sodium succinate were used as substrates. The cultivation at 37 degrees C caused a sharp increase in the growth rate which, however, decreased at the end of the experiment (96th hour of cultivation), and certain stimulation of growth was observed on D-raffinose-containing medium. The growth of cells cultivated at 4 degrees C using L-rhamnose as substrate started after a lag-phase of 24 hours, which was better expressed for serovars 0:8 and 0:9. The results reported could be applied to experiments, using methods of isolation of Yersinia enterocolitica from different sources after cultivation at 4 degrees C on L-rhamnose-containing media.