Y Germani, S Insisiengmay, P Boupha, B Mixap, T Khouan, G Le Gonidec
{"title":"[Characterization of the enterotoxigenic strains of Escherichia coli isolated from children with diarrhea in Popular Democratic Republic Lao].","authors":"Y Germani, S Insisiengmay, P Boupha, B Mixap, T Khouan, G Le Gonidec","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Enterotoxigenic Escherichia coli (ETEC) of infant origin from the Popular Democratic Republic Lao were characterized with respect to there 0 serogroups, biotypes, anti-bioresistances, fimbrial antigens and types of enterotoxins produced. Enterotoxin production was determined by the suckling mice assay, competitive GM1-erythroassay, and cell cultures (CHOK1 and Y1). The presence of genes encoding for the enterotoxins was determined by colony hybridization by using radioactive DNA probes. Profile plasmids from ETEC strains were studied. The plasmids encoding for heat-labile enterotoxin were studied with an acetyl-aminofluorene modified probe.</p>","PeriodicalId":9297,"journal":{"name":"Bulletin de la Societe de pathologie exotique et de ses filiales","volume":"82 4","pages":"465-75"},"PeriodicalIF":0.0000,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin de la Societe de pathologie exotique et de ses filiales","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Enterotoxigenic Escherichia coli (ETEC) of infant origin from the Popular Democratic Republic Lao were characterized with respect to there 0 serogroups, biotypes, anti-bioresistances, fimbrial antigens and types of enterotoxins produced. Enterotoxin production was determined by the suckling mice assay, competitive GM1-erythroassay, and cell cultures (CHOK1 and Y1). The presence of genes encoding for the enterotoxins was determined by colony hybridization by using radioactive DNA probes. Profile plasmids from ETEC strains were studied. The plasmids encoding for heat-labile enterotoxin were studied with an acetyl-aminofluorene modified probe.
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