Detection and isolation of a new member of Burkholderiaceae-related endofungal bacteria from Saksenaea boninensis sp. nov., a new thermotolerant fungus in Mucorales.

IF 5.2 1区 生物学 Q1 MYCOLOGY Ima Fungus Pub Date : 2023-11-23 DOI:10.1186/s43008-023-00129-2
Yusuke Takashima, Kohei Yamamoto, Yousuke Degawa, Yong Guo, Tomoyasu Nishizawa, Hiroyuki Ohta, Kazuhiko Narisawa
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Abstract

Thermotolerance in Mucorales (Mucoromycotina) is one of the factors to be opportunistic pathogens, causing mucormycosis. Among thermotolerant mucoralean fungi, Burkholderiaceae-related endobacteria (BRE) are rarely found and the known range of hosts is limited to Rhizopus spp. The phylogenetic divergence of BRE has recently expanded in other fungal groups such as Mortierellaceae spp. (Mortierellomycotina); however, it remains unexplored in Mucorales. Here, we found a thermotolerant mucoralean fungus obtained from a litter sample collected from Haha-jima Island in the Ogasawara (Bonin) Islands, Japan. The fungus was morphologically, phylogenetically, and physiologically characterized and proposed as a new species, Saksenaea boninensis sp. nov. Besides the fungal taxonomy, we also found the presence of BRE in isolates of this species by diagnostic PCR amplification of the 16S rRNA gene from mycelia, fluorescence microscopic observations, and isolation of the bacterium in pure culture. Phylogenetic analysis of the 16S rRNA gene of BRE revealed that it is distinct from all known BRE. The discovery of a culturable BRE lineage in the genus Saksenaea will add new insight into the evolutional origin of mucoralean fungus-BRE associations and emphasize the need to pay more attention to endofungal bacteria potentially associated with isolates of thermotolerant mucoralean fungi causing mucormycosis.

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Mucorales耐高温真菌Saksenaea boninensis sp. nov.中burkholderacae相关内真菌新成员的检测与分离。
毛霉菌属(Mucoromycotina)的耐热性是条件致病菌引起毛霉病的因素之一。在耐高温的粘膜真菌中,burkholderacae -related endobacteria (BRE)很少被发现,已知的宿主范围仅限于根霉属(Rhizopus spp), BRE的系统发育分化最近在其他真菌类群如Mortierellaceae spp (Mortierellomycotina);然而,它在Mucorales仍未被探索。在这里,我们从日本小笠原(小笠原)群岛哈哈哈岛的凋落物样本中发现了一种耐热的粘膜真菌。在形态学、系统发育和生理学上对该真菌进行了鉴定,并提出作为一个新种,Saksenaea boninensis sp. 11 .除真菌分类外,我们还通过菌丝16S rRNA基因的诊断性PCR扩增、荧光显微镜观察和纯培养分离发现该菌株存在BRE。对BRE的16S rRNA基因进行系统发育分析,发现它与所有已知的BRE不同。在Saksenaea属中可培养的BRE谱系的发现将为mucoralean真菌-BRE关联的进化起源提供新的见解,并强调需要更多地关注可能与引起毛霉病的耐高温mucoralean真菌分离株相关的内真菌细菌。
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来源期刊
Ima Fungus
Ima Fungus Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
11.00
自引率
3.70%
发文量
18
审稿时长
20 weeks
期刊介绍: The flagship journal of the International Mycological Association. IMA Fungus is an international, peer-reviewed, open-access, full colour, fast-track journal. Papers on any aspect of mycology are considered, and published on-line with final pagination after proofs have been corrected; they are then effectively published under the International Code of Nomenclature for algae, fungi, and plants. The journal strongly supports good practice policies, and requires voucher specimens or cultures to be deposited in a public collection with an online database, DNA sequences in GenBank, alignments in TreeBASE, and validating information on new scientific names, including typifications, to be lodged in MycoBank. News, meeting reports, personalia, research news, correspondence, book news, and information on forthcoming international meetings are included in each issue
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