[Lipopolysaccharide-containing cytoplasmic membranes as immunostimulants of peritoneal macrophages].

Abstract

It was established that cytoplasmic membranes from the stable L-forms of E. coli WF+ induced a 4-5 fold increase of the number of peritoneal exudative cells in mice after single i.p. inoculation. The animals treated with membranes reacted with 4-5 fold higher number of these cells after an i.p. infection by parent form cells or L-form cells, as compared with the reaction of infected, nontreated by membranes mice. The macrophage bactericidal activity was 6-10 fold increased. Using electron microscopy, it was established that the phagosomes containing membranes and their remnants were localized in the peripheral part of the cytoplasm or near the nucleus, without neighbouring lysosomes during the 24-hour interaction. Single cases of phagolysosome fusion were observed.