An improved method for studying grass leaf epidermis.

J D Theunissen
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引用次数: 3

Abstract

Leaf epidermis of grasses is elaborate and important in the systematics of the Poaceae at subfamily and genus level. Most available techniques used in preparing leaf epidermis for microscopic studies are time-consuming and often produce preparations inadequate for studying histological detail. A combination of the hand scraping and maceration methods with modifications is proposed in this paper to prepare epidermal peels comparatively rapidly. One epidermal layer was scraped off and the mesophyll tissue removed from the epidermis to be studied by maceration in HNO3. The recovered epidermal peel was neutralized in NaOH and stained with malachite green or safranin O. Preparations made by this technique are suitable for studies of epidermal features, measurements of special structures and determinations of trichome indices. This method has been used in a study investigating intraspecific variation in southern African pasture grasses.

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一种改进的草叶表皮研究方法。
禾本科植物的叶表皮在禾本科亚科和属的系统分类中占有重要地位。用于制备叶表皮进行显微研究的大多数现有技术都是耗时的,并且通常产生的制剂不足以研究组织学细节。本文提出了一种将手刮法和浸泡法结合并进行修饰的方法,以相对快速地制备表皮去皮。刮去一层表皮,用HNO3浸渍法去除表皮上的叶肉组织。将回收的表皮果皮用NaOH中和,并用孔雀石绿或红花红o染色。该技术制备的产品适用于表皮特征的研究、特殊结构的测量和毛状体指数的测定。这种方法已被用于研究非洲南部牧草种内变异。
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