Detection and Analysis of Blood Dexmedetomidine in Drug-Facilitated Cases.

Journal of Forensic Medicine Pub Date : 2023-10-25 DOI:10.12116/j.issn.1004-5619.2022.320306

Wen-Ya Zhai, Li-Na Wu, Shuo Yang, Bao-Hua Shen, Yan Shi

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Abstract

Objectives: To establish a simple and rapid qualitative and quantitative detection method of dexmedetomidine in blood.

Methods: Blood was separated on the Allure PFP Propyl liquid chromatography column with isocratic elution after it was precipitated by acetonitrile and filtered. Qualitative and quantitative analysis of dexmedetomidine was performed using positive ion scan mode and multi-reaction monitoring mode.

Results: The limit of detection of dexmedetomidine in blood was 0.2 ng/mL and the limit of quantification was 0.5 ng/mL. The linearity of the method was good in the range of 0.5-1 000 ng/mL, and the correlation coefficient was greater than 0.99. The accuracy of the method was 90.34%-112.67% and the extraction recovery was 50.05%-91.08%, with no significant matrix effect.

Conclusions: This method is simple, selective and suitable for the qualitative and quantitative analysis of dexmedetomidine in blood, which can provide a reference for drug-facilitated cases involving dexmedetomidine.

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药物依赖性病例血中右美托咪定的检测与分析。

目的:建立一种简便、快速的血液中右美托咪定的定性、定量检测方法。方法:血液经乙腈沉淀过滤后，在Allure PFP丙基液相色谱柱上等压洗脱分离。采用正离子扫描模式和多反应监测模式对右美托咪定进行定性和定量分析。结果:血液中右美托咪定的检出限为0.2 ng/mL，定量限为0.5 ng/mL。该方法在0.5 ~ 1000 ng/mL范围内线性良好，相关系数大于0.99。方法精密度为90.34% ~ 112.67%，提取回收率为50.05% ~ 91.08%，无显著基质效应。结论:该方法简便、选择性好，适用于血液中右美托咪定的定性和定量分析，可为涉及右美托咪定的药物依赖型病例提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Forensic Medicine

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