Modulation of semaphorin 3C & 4D expression in cancerous tissues from individuals with laryngeal squamous cell carcinoma.

IF 2.7 4区医学 Q3 IMMUNOLOGY Indian Journal of Medical Research Pub Date : 2023-10-01 Epub Date: 2023-09-25 DOI:10.4103/ijmr.ijmr_3148_21

Marzieh Sajadian, Bijan Khademi, Leila Moinzadeh, Abbas Ghaderi, Abdollah Jafarzadeh, Mahboobeh Razmkhah

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Abstract

Background objectives: Semaphorins were initially characterized as axon guidance factors but were subsequently implicated in the regulation of immune responses, angiogenesis, organ formation and a variety of other physiological and developmental functions. Various semaphorins enhance or inhibit tumour progression through different mechanisms. The objective of this study was to assess the expression of various semaphorins and vascular endothelial growth factor (VEGF) gene transcripts as well as the serum level of Sema3A in individuals with laryngeal squamous cell carcinoma (LSCC).

Methods: Tissue expression of Sema3A, Sema3C, Sema4D, Sema6D and VEGF was determined in both tumour tissues and tissues around the tumour from 30 individuals with pathologically confirmed LSCC using quantitative real-time PCR. Furthermore, the serum level of Sema3A in these individuals was assessed using enzyme-linked immunosorbent assay.

Results: Sema3C gene transcript showed a significant increase (P=0.001), while Sema4D was observed with a significant decrease in tumour samples compared to non-tumoural tissues (P≤0.01). The expression of the Sema3C gene was found to be associated with the stage of LSCC tumour as it was statistically significant for tumours with stage IV (P<0.01). The serum level of Sema3A was not found to be significant between cases and controls.

Interpretation conclusions: Increased expression of Sema3C but decreased expression of Sema4D in tumour tissue of LSCC may introduce these two growth factors as crucial mediators orchestrating tumour growth in individuals with LSCC. This result could open a new vision for the treatment of this malignancy.

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信号蛋白3C和4D在喉癌组织中的表达调控。

背景目的:信号蛋白最初被认为是轴突引导因子，但随后涉及免疫反应、血管生成、器官形成和各种其他生理和发育功能的调节。不同的信号素通过不同的机制促进或抑制肿瘤的进展。本研究的目的是评估喉鳞状细胞癌(LSCC)患者中各种信号素和血管内皮生长因子(VEGF)基因转录物的表达以及血清中Sema3A的水平。方法:采用实时荧光定量PCR检测30例病理证实的LSCC患者肿瘤组织及肿瘤周围组织中Sema3A、Sema3C、Sema4D、Sema6D和VEGF的组织表达。此外，使用酶联免疫吸附法评估这些个体的血清Sema3A水平。结果:Sema3C基因转录量显著增加(P=0.001)， Sema4D基因转录量在肿瘤组织中较非肿瘤组织显著减少(P≤0.01)。研究发现，Sema3C基因的表达与LSCC肿瘤分期相关，在IV期肿瘤中具有统计学意义(p)。解释结论:在LSCC肿瘤组织中，Sema3C基因的表达增加，而Sema4D基因的表达减少，可能使这两种生长因子成为协调LSCC个体肿瘤生长的关键介质。这一结果为肿瘤的治疗开辟了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Indian Journal of Medical Research 医学-免疫学

CiteScore

5.80

自引率

2.40%

发文量

191

审稿时长

3-8 weeks

期刊介绍： The Indian Journal of Medical Research (IJMR) [ISSN 0971-5916] is one of the oldest medical Journals not only in India, but probably in Asia, as it started in the year 1913. The Journal was started as a quarterly (4 issues/year) in 1913 and made bimonthly (6 issues/year) in 1958. It became monthly (12 issues/year) in the year 1964.