Ready-to-use nanopore platform for label-free small molecule quantification: Ethanolamine as first example

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2023-11-23 DOI:10.1016/j.nano.2023.102724
Isabel Quint MSc , Jonathan Simantzik MSc , Lars Kaiser PhD , Stefan Laufer PhD , Rene' Csuk PhD , David Smith PhD , Matthias Kohl PhD , Hans-Peter Deigner PhD
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Abstract

In recent decades, nanopores have become a promising diagnostic tool. Protein and solid-state nanopores are increasingly used for both RNA/DNA sequencing and small molecule detection. The latter is of great importance, as their detection is difficult or expensive using available methods such as HPLC or LC-MS. DNA aptamers are an excellent detection element for sensitive and specific detection of small molecules. Herein, a method for quantifying small molecules using a ready-to-use sequencing platform is described.

Taking ethanolamine as an example, a strand displacement assay is developed in which the target-binding aptamer is displaced from the surface of magnetic particles by ethanolamine. Non-displaced aptamer and thus the ethanolamine concentration are detected by the nanopore system and can be quantified in the micromolar range using our in-house developed analysis software. This method is thus the first to describe a label-free approach for the detection of small molecules in a protein nanopore system.

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现成的纳米孔平台,用于无标记的小分子定量:乙醇胺为第一个例子。
近几十年来,纳米孔已成为一种很有前途的诊断工具。蛋白质和固态纳米孔越来越多地用于RNA/DNA测序和小分子检测。后者非常重要,因为使用HPLC或LC-MS等现有方法检测它们困难或昂贵。DNA适体是一种灵敏、特异的小分子检测元件。本文描述了一种利用即用型测序平台定量小分子的方法。以乙醇胺为例,开发了一种链置换法,其中目标结合适体被乙醇胺从磁性颗粒表面置换。通过纳米孔系统检测非位移适配体和乙醇胺浓度,并可以使用我们内部开发的分析软件在微摩尔范围内进行量化。因此,该方法首次描述了一种用于检测蛋白质纳米孔系统中的小分子的无标记方法。
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来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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