Resazurin microplate test method for rapid determination of colistin resistance in carbapenem-resistant Acinetobacter baumanii isolates.

Laboratory medicine Pub Date : 2024-05-02 DOI:10.1093/labmed/lmad099

Kubra Yildirim, Ece Simsek, Orhan Kocak, Serhat Bozkurt, Ozlem Koyuncu Ozyurt, Ahmet Yilmaz Coban

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Abstract

Background: Colistin-resistant Acinetobacter baumannii isolates are extremely important pathogens for hospital-acquired infections.

Objective: To investigate the effectiveness of the resazurin microplate assay (REMA) for the rapid determination of colistin resistance.

Methods: Susceptibility for colistin was investigated in vitro by the broth microdilution method (BMD) and the resazurin microplate assay (REMA) on 106 carbapenem-resistant Acinetobacter baumannii isolates.

Results: The results of both test methods were compared, and the categorical agreement between them was found to be 100%. No minor, major, or very major discrepancy was observed between the 2 methods.

Conclusions: The most important advantages of REMA are that the results are obtained within 6 hours compared to the reference method, that it is easy to evaluate because it is colorimetric, and that the susceptibility result can be reported to the clinician on the same day as bacterial identification.

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耐碳青霉烯鲍曼不动杆菌分离株粘菌素耐药性的瑞唑脲微孔板试验方法。

背景:耐粘菌素鲍曼不动杆菌分离株是医院获得性感染极其重要的病原体。目的:探讨瑞祖菌素微孔板法(REMA)快速测定粘菌素耐药性的有效性。方法:采用肉汤微量稀释法(BMD)和瑞祖林微孔板法(REMA)对106株耐碳青霉烯鲍曼不动杆菌进行体外对粘菌素的敏感性研究。结果:对两种检测方法的结果进行比较，两种方法的绝对一致性为100%。两种方法之间没有观察到轻微、严重或非常严重的差异。结论:与参考方法相比，REMA最重要的优点是可在6小时内获得结果，由于是比色法，易于评价，并且药敏结果可在细菌鉴定当天报告给临床医生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Laboratory medicine

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