[¹⁸F]FDG PET-MR characterization of aortitis in the IL1rn^-/- mouse model of giant-cell arteritis.

IF 3.1 3区医学 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING EJNMMI Research Pub Date : 2023-11-29 DOI:10.1186/s13550-023-01039-5

Samuel Deshayes, Caroline Baugé, Pierre-Antoine Dupont, Christophe Simard, Hanan Rida, Hubert de Boysson, Alain Manrique, Achille Aouba

{"title":"[18F]FDG PET-MR characterization of aortitis in the IL1rn-/- mouse model of giant-cell arteritis.","authors":"Samuel Deshayes, Caroline Baugé, Pierre-Antoine Dupont, Christophe Simard, Hanan Rida, Hubert de Boysson, Alain Manrique, Achille Aouba","doi":"10.1186/s13550-023-01039-5","DOIUrl":null,"url":null,"abstract":"Background: Metabolic imaging is routinely used to demonstrate aortitis in patients with giant-cell arteritis. We aimed to investigate the preclinical model of aortitis in BALB/c IL1rn-/- mice using [18F]fluorodeoxyglucose ([18F]FDG) positron emission tomography-magnetic resonance (PET-MR), gamma counting and immunostaining. We used 15 first-generation specific and opportunistic pathogen-free (SOPF) 9-week-old IL1rn-/- mice, 15 wild-type BALB/cAnN mice and 5 s-generation specific pathogen-free (SPF) 9-week-old IL1rn-/-. Aortic [18F]FDG uptake was assessed as the target-to-background ratio (TBR) using time-of-flight MR angiography as vascular landmarks.Results: [18F]FDG uptake measured by PET or gamma counting was similar in the first-generation SOPF IL1rn-/- mice and the wild-type group (p > 0.05). However, the first-generation IL1rn-/- mice exhibited more interleukin-1β (p = 0.021)- and interleukin-6 (p = 0.019)-positive cells within the abdominal aorta than the wild-type mice. In addition, the second-generation SPF group exhibited significantly higher TBR (p = 0.0068) than the wild-type mice on the descending thoracic aorta, unlike the first-generation SOPF IL1rn-/- mice.Conclusions: In addition to the involvement of interleukin-1β and -6 in IL1rn-/- mouse aortitis, this study seems to validate [18F]FDG PET-MR as a useful tool for noninvasive monitoring of aortitis in this preclinical model.","PeriodicalId":11611,"journal":{"name":"EJNMMI Research","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687326/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"EJNMMI Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13550-023-01039-5","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Metabolic imaging is routinely used to demonstrate aortitis in patients with giant-cell arteritis. We aimed to investigate the preclinical model of aortitis in BALB/c IL1rn^-/- mice using [¹⁸F]fluorodeoxyglucose ([¹⁸F]FDG) positron emission tomography-magnetic resonance (PET-MR), gamma counting and immunostaining. We used 15 first-generation specific and opportunistic pathogen-free (SOPF) 9-week-old IL1rn^-/- mice, 15 wild-type BALB/cAnN mice and 5 s-generation specific pathogen-free (SPF) 9-week-old IL1rn^-/-. Aortic [¹⁸F]FDG uptake was assessed as the target-to-background ratio (TBR) using time-of-flight MR angiography as vascular landmarks.

Results: [¹⁸F]FDG uptake measured by PET or gamma counting was similar in the first-generation SOPF IL1rn^-/- mice and the wild-type group (p > 0.05). However, the first-generation IL1rn^-/- mice exhibited more interleukin-1β (p = 0.021)- and interleukin-6 (p = 0.019)-positive cells within the abdominal aorta than the wild-type mice. In addition, the second-generation SPF group exhibited significantly higher TBR (p = 0.0068) than the wild-type mice on the descending thoracic aorta, unlike the first-generation SOPF IL1rn^-/- mice.

Conclusions: In addition to the involvement of interleukin-1β and -6 in IL1rn^-/- mouse aortitis, this study seems to validate [¹⁸F]FDG PET-MR as a useful tool for noninvasive monitoring of aortitis in this preclinical model.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

[18F] il - 1rn-/-小鼠巨细胞动脉炎模型的FDG PET-MR表征。

背景:代谢成像通常用于巨细胞动脉炎患者的动脉炎诊断。我们采用[18F]氟脱氧葡萄糖([18F]FDG)正电子发射断层扫描-磁共振(PET-MR)、伽马计数和免疫染色研究BALB/c IL1rn-/-小鼠的临床前主动脉炎模型。我们使用15只第一代特异性和机会性无病原体(SOPF) 9周龄IL1rn-/-小鼠，15只野生型BALB/cAnN小鼠和5只s代特异性无病原体(SPF) 9周龄IL1rn-/-小鼠。使用飞行时间MR血管造影作为血管标志，以靶本比(TBR)评估主动脉[18F]FDG摄取。结果:[18F]第一代SOPF IL1rn-/-小鼠与野生型组FDG摄食量相近(p > 0.05)。然而，与野生型小鼠相比，第一代IL1rn-/-小鼠在腹主动脉内表现出更多的白细胞介素-1β (p = 0.021)和白细胞介素-6 (p = 0.019)阳性细胞。此外，与第一代SOPF IL1rn-/-小鼠不同，第二代SPF组在胸降主动脉上的TBR显著高于野生型小鼠(p = 0.0068)。结论:除了白细胞介素-1β和-6参与IL1rn-/-小鼠主动脉炎外，本研究似乎验证了[18F]FDG PET-MR在临床前模型中作为无创监测主动脉炎的有用工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

EJNMMI Research RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING&nb-

CiteScore

5.90

自引率

3.10%

发文量

审稿时长

13 weeks

期刊介绍： EJNMMI Research publishes new basic, translational and clinical research in the field of nuclear medicine and molecular imaging. Regular features include original research articles, rapid communication of preliminary data on innovative research, interesting case reports, editorials, and letters to the editor. Educational articles on basic sciences, fundamental aspects and controversy related to pre-clinical and clinical research or ethical aspects of research are also welcome. Timely reviews provide updates on current applications, issues in imaging research and translational aspects of nuclear medicine and molecular imaging technologies. The main emphasis is placed on the development of targeted imaging with radiopharmaceuticals within the broader context of molecular probes to enhance understanding and characterisation of the complex biological processes underlying disease and to develop, test and guide new treatment modalities, including radionuclide therapy.