Effects of paclitaxel before vitrification on the nuclear maturation and development of immature porcine oocytes.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2023-09-01

M Tone, R Ukyo, S H Sakamoto, K Hemmi, I Kokayashi, Y Tsuzuki

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Abstract

Background: Cryopreservation of porcine oocytes is difficult compared with other species and immature oocytes particularly so compared to the meiotic stage.

Objective: To evaluate the efficacy of a pretreatment with 1 micromole per L paclitaxel (PTX, 30 min exposure) before vitrification to promote the maturation of porcine immature oocytes.

Materials and methods: Cumulus cell-enclosed oocytes (COs) aspirated from porcine ovaries were divided into three groups: i) non-pretreated with PTX and non-vitrified group (control group); ii) pretreated with PTX and vitrified group (PTX-V group); and iii) non-pretreated with PTX and vitrified group (nPTX-V group).

Results: The nuclear maturation rate up to the preovulatory stage was significantly lower (P < 0.05) in the nPTX-V group than in the control group, but was similar in the PTX-V and control groups. No significant differences were observed in viability assessed by a normal CO morphology and the embryonic development of oocytes activated by the parthenogenetic stimulation between the PTX-V and control groups, but not the non-PTX-V group.

Conclusion: PTX may promote the maturation of vitrified porcine immature oocytes. Doi.org/10.54680/fr23510110812.

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玻璃化前紫杉醇对未成熟猪卵母细胞核成熟和发育的影响。

背景:猪卵母细胞的低温保存与其他物种和未成熟卵母细胞相比是困难的，特别是与减数分裂阶段相比。目的:评价玻璃化前1微摩尔/ L紫杉醇(PTX，暴露30 min)预处理促进猪未成熟卵母细胞成熟的效果。材料与方法:取猪卵巢积云细胞封闭卵母细胞(COs)分为3组:1)未经PTX预处理和非玻璃化处理组(对照组);ii) PTX预处理和玻璃化组(PTX- v组);iii)未经PTX预处理和玻璃化组(nPTX-V组)。结果:nPTX-V组至排卵期核成熟率显著低于对照组(P < 0.05)，而PTX-V组与对照组无明显差异。在正常CO形态和孤雌生殖刺激激活的卵母细胞的胚胎发育方面，PTX-V组和对照组之间没有显著差异，但非PTX-V组没有。结论:PTX可促进玻璃化猪未成熟卵母细胞的成熟。Doi.org/10.54680/fr23510110812。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.