PERSPECTIVE: Potential and reality of cryopreserving somatic cells of wild felids for conservation.

IF 1 4区 生物学 Q3 BIOLOGY Cryo letters Pub Date : 2023-09-01
L L Vieira Rodrigues, A F Pereira
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Abstract

The loss of biodiversity caused by anthropogenic actions is also a reality for the members of the Felidae family. Except for the domestic cat, all felid species have some degree of threat of extinction in their natural habitat. For this reason, felids have been included in conservation-related studies. This scenario has aroused increasing interest in the formation of somatic cell banks, which when efficiently implemented can be used in preservation strategies for the species. Nevertheless, one of the important steps in the formation of these banks is the understanding of the technical principles and variations involved in cryopreservation techniques, especially because cryopreservation increases the possibilities for Assisted Reproduction Technologies (ARTs) by making the use of biological materials independent of time and space. In wild felids, several species already have promising results in the formation of somatic cell banks, and studies aimed at better viability rates have been constantly proposed, as well as new species have been studied. In some species, aspects involved in successful cryopreservation are already well defined, and slow freezing associated with cryoprotectant solutions composed of intra- and extracellular substances is the most useful approach. The aim of this review was to present the main parameters involved in the elaboration of a somatic cell cryopreservation protocol and their effects, as well as to address the main results achieved for different wild felids. Doi.org/10.54680/fr23510110112.

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展望:野生猫科动物体细胞低温保存的潜力与现实。
人类活动造成的生物多样性丧失也是Felidae科成员面临的现实。除家猫外,所有的猫科动物在其自然栖息地都有一定程度的灭绝威胁。因此,猫科动物已被纳入保护相关研究。这种情况引起了人们对体细胞库形成的兴趣,当体细胞库有效实施时,可以用于物种的保护策略。然而,这些干细胞库形成的重要步骤之一是对低温保存技术的技术原理和变化的理解,特别是因为低温保存通过使用独立于时间和空间的生物材料增加了辅助生殖技术(ARTs)的可能性。在野生猫科动物中,一些物种已经在形成体细胞库方面取得了有希望的结果,并且不断提出旨在提高存活率的研究,以及对新物种的研究。在一些物种中,成功低温保存涉及的方面已经很好地定义了,与细胞内和细胞外物质组成的冷冻保护剂溶液相关联的缓慢冷冻是最有用的方法。本综述的目的是介绍体细胞冷冻保存方案的主要参数及其影响,并讨论在不同野生田中取得的主要结果。Doi.org/10.54680/fr23510110112。
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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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