Prevalence of multidrug-resistant Gram-negative bacteria from blood cultures and rapid detection of beta-lactamase-encoding genes by multiplex PCR assay.

IF 1.7 Q3 INFECTIOUS DISEASES GERMS Pub Date : 2022-12-31 eCollection Date: 2022-12-01 DOI:10.18683/germs.2022.1349

Sanja Zornic, Bojana Lukovic, Ivana Petrovic, Aleksandra Jencic

{"title":"Prevalence of multidrug-resistant Gram-negative bacteria from blood cultures and rapid detection of beta-lactamase-encoding genes by multiplex PCR assay.","authors":"Sanja Zornic, Bojana Lukovic, Ivana Petrovic, Aleksandra Jencic","doi":"10.18683/germs.2022.1349","DOIUrl":null,"url":null,"abstract":"Introduction: This study aimed to determine the prevalence of multidrug-resistant Gram-negative bacteria (GNB) from blood cultures in a tertiary-care hospital and the multiplex PCR assay's ability to detect resistance genes.Methods: A total of 388 GNB isolates obtained from hospitalized patients between November 2019 and November 2021 were included in the study. Antimicrobial susceptibility testing was done by VITEK 2 system and broth microdilution method. Beta-lactamase-encoding genes were detected by multiplex PCR assays, BioFire-Blood Culture Identification 2 (BCID2) panel (bioMérieux, France). Extended-spectrum beta-lactamases (ESBLs) were detected phenotypically with VITEK AST-GN71 card (bioMérieux, France). The isolates of GNB were classified into multidrug-resistant, extensively-drug-resistant, and pandrug-resistant categories, and their prevalence and distribution in different wards, including coronavirus diseases 2019 (COVID-19) intensive care units (ICU), were calculated.Results: Results revealed that all isolates of Acinetobacter baumannii and Pseudomonas aeruginosa were multidrug-resistant as well as 91.6% of Enterobacter cloacae, 80.6% of Proteus mirabilis, and 76.1% of Klebsiella pneumoniae, respectively. In fermentative bacteria, blaOXA-48-like (58.1%), blaNDM (16.1%), blaKPC (9.7%) and blaVIM (6.5%) genes were detected. More than half of Enterobacter cloacae (58.3%) and Klebsiella pneumoniae (53.7%) produced ESBLs. Among non-fermenters, the blaNDM gene was carried by 55% of Pseudomonas aeruginosa and 19.5% of Acinetobacter baumannii. In the COVID-19 ICU, Acinetobacter baumannii was the most common isolate (86.1%).Conclusions: This study revealed high proportions of multidrug-resistant blood isolates and various underlying resistance genes in Gram-negative strains. The BCID2 panel seems to be helpful for the detection of the most prevalent resistance genes of fermentative bacteria.","PeriodicalId":45107,"journal":{"name":"GERMS","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660225/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"GERMS","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18683/germs.2022.1349","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/12/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: This study aimed to determine the prevalence of multidrug-resistant Gram-negative bacteria (GNB) from blood cultures in a tertiary-care hospital and the multiplex PCR assay's ability to detect resistance genes.

Methods: A total of 388 GNB isolates obtained from hospitalized patients between November 2019 and November 2021 were included in the study. Antimicrobial susceptibility testing was done by VITEK 2 system and broth microdilution method. Beta-lactamase-encoding genes were detected by multiplex PCR assays, BioFire-Blood Culture Identification 2 (BCID2) panel (bioMérieux, France). Extended-spectrum beta-lactamases (ESBLs) were detected phenotypically with VITEK AST-GN71 card (bioMérieux, France). The isolates of GNB were classified into multidrug-resistant, extensively-drug-resistant, and pandrug-resistant categories, and their prevalence and distribution in different wards, including coronavirus diseases 2019 (COVID-19) intensive care units (ICU), were calculated.

Results: Results revealed that all isolates of Acinetobacter baumannii and Pseudomonas aeruginosa were multidrug-resistant as well as 91.6% of Enterobacter cloacae, 80.6% of Proteus mirabilis, and 76.1% of Klebsiella pneumoniae, respectively. In fermentative bacteria, bla_OXA-48-like (58.1%), bla_NDM (16.1%), bla_KPC (9.7%) and bla_VIM (6.5%) genes were detected. More than half of Enterobacter cloacae (58.3%) and Klebsiella pneumoniae (53.7%) produced ESBLs. Among non-fermenters, the bla_NDM gene was carried by 55% of Pseudomonas aeruginosa and 19.5% of Acinetobacter baumannii. In the COVID-19 ICU, Acinetobacter baumannii was the most common isolate (86.1%).

Conclusions: This study revealed high proportions of multidrug-resistant blood isolates and various underlying resistance genes in Gram-negative strains. The BCID2 panel seems to be helpful for the detection of the most prevalent resistance genes of fermentative bacteria.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

血液培养中多重耐药革兰氏阴性菌的流行及多重PCR快速检测β -内酰胺酶编码基因。

本研究旨在确定三级医院血液培养物中多重耐药革兰氏阴性菌(GNB)的流行情况，以及多重PCR检测耐药基因的能力。方法:纳入2019年11月至2021年11月住院患者中分离到的388株GNB。采用VITEK 2系统和微量肉汤稀释法进行药敏试验。β -内酰胺酶编码基因在BioFire-Blood Culture Identification 2 (bid2) panel (biomacrieux, France)上进行多重PCR检测。广谱β -内酰胺酶(ESBLs)用VITEK AST-GN71卡(biomacrieux, France)进行表型检测。将GNB分离株分为多重耐药、广泛耐药和大耐药三类，并计算其在包括2019冠状病毒病(COVID-19)重症监护病房(ICU)在内的不同病房的流行和分布情况。结果:鲍曼不动杆菌和铜绿假单胞菌均耐多药，阴沟肠杆菌、奇迹变形杆菌和肺炎克雷伯菌的耐多药率分别为91.6%、80.6%和76.1%。发酵菌中检测到blaoxa -48样(58.1%)、blaNDM(16.1%)、blaKPC(9.7%)和blaVIM(6.5%)基因。超过一半的阴沟肠杆菌(58.3%)和肺炎克雷伯菌(53.7%)产生ESBLs。在非发酵菌中，55%的铜绿假单胞菌和19.5%的鲍曼不动杆菌携带blaNDM基因。在COVID-19 ICU中，鲍曼不动杆菌是最常见的分离物(86.1%)。结论:本研究揭示了革兰氏阴性菌株中高比例的多药耐药血分离株和多种潜在耐药基因。bbcid2小组似乎有助于检测最普遍的耐药基因的发酵细菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

GERMS INFECTIOUS DISEASES-

CiteScore

2.80

自引率

5.00%

发文量