Regulation of ROS/inflammasome Axis is Essential for Cardiac Regeneration in Aging Rats Receiving Transplantation of Mesenchymal Stem Cells.

Wei-Syun Hu, Jing-Yi Chen, Wei-Yu Liao, Chin-Hsien Chang, Tung-Sheng Chen
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Abstract

Background: Aging is a biological and gradual deterioration of function in living organisms. Aging is one of the risk factors for heart disease.

Objective: Although mesenchymal stem cell transplantation shows potential in heart disease treatment, the relationship between stem cell-based therapy and oxidative stress/inflammasome axis regulation remains unclear. This study hypothesized that intervention of stem cells showed protective effect on heart aging induced by D-galactose through regulation of oxidative stress/inflammasome axis.

Methods: An aging animal model was designed to test the above hypothesis. Experimental animals were divided into three groups, including Sham, D-gal (aging rats induced by d-galactose), and D-gal+WJSC (aging rats receiving mesenchymal stem cells).

Results: Compared to the Sham, the experimental results indicate that structural alteration (HE stain and Masson's Trichrome stain), oxidative stress elevation (increase of TBARS level, expression of gp-91 and suppression of Sirt-1 as well as SOD2), increase of aging marker p53, suppression of cardiogenesis marker Troponin T, and inflammasome related protein markers expression (NLRP3, caspase-1 and IL-1 beta) were significantly observed in D-gal. In contrast, all pathological pathways were significantly improved in D-gal+WJSC when compared to D-gal. In addition, migration of stem cells to aging heart tissues was observed in the D-gal+WJSC group.

Conclusion: These findings suggest that mesenchymal stem cell transplantation effectively ameliorates aging hearts through oxidative stress/inflammasome axis regulation. The results from this study provide clinical potential for stem cell-based therapy in the treatment of aging hearts.

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ROS/炎性体轴的调节对接受间充质干细胞移植的衰老大鼠心脏再生至关重要。
背景:衰老是生物体功能逐渐退化的生物学过程。衰老是心脏病的危险因素之一。目的:尽管间充质干细胞移植在心脏病治疗中显示出潜力,但干细胞治疗与氧化应激/炎症小体轴调节之间的关系尚不清楚。本研究假设干细胞干预通过调节氧化应激/炎性体轴对d -半乳糖诱导的心脏衰老具有保护作用。方法:设计衰老动物模型验证上述假设。实验动物分为Sham、D-gal (d-半乳糖诱导的衰老大鼠)和D-gal+WJSC(接受间充质干细胞的衰老大鼠)三组。结果:与Sham相比,实验结果显示D-gal细胞结构改变(HE染色和Masson’s Trichrome染色)、氧化应激升高(TBARS水平升高、gp-91表达、Sirt-1和SOD2抑制)、衰老标志物p53升高、心肌生成标志物Troponin T抑制、炎性小体相关蛋白标志物NLRP3、caspase-1和IL-1 β表达显著升高。与D-gal相比,D-gal+WJSC的所有病理通路都得到了显著改善。此外,在D-gal+WJSC组中观察到干细胞向老化心脏组织的迁移。结论:这些发现提示间充质干细胞移植通过氧化应激/炎症小体轴调节有效改善老化心脏。这项研究的结果为干细胞治疗老化心脏提供了临床潜力。
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