The presence of glutathione peroxidase 8 (GPx8) in rat male genital organs.

IF 1.5 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Bratislava Medical Journal-Bratislavske Lekarske Listy Pub Date : 2024-01-01 DOI:10.4149/BLL_2024_008
Jozef Mihalik, Peter Solar, Kristina Curgali, Martin Svana, Andrea Krehelova, Marko Vrzgula, Viera Almasiova, Ingrid Hodorova
{"title":"The presence of glutathione peroxidase 8 (GPx8) in rat male genital organs.","authors":"Jozef Mihalik, Peter Solar, Kristina Curgali, Martin Svana, Andrea Krehelova, Marko Vrzgula, Viera Almasiova, Ingrid Hodorova","doi":"10.4149/BLL_2024_008","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>The aim of our work was to determine the presence of GPx8, the latest discovered member of glutathione peroxidase family, in rat male genital organs.</p><p><strong>Background: </strong>The oxidative stress is considered as one of the most important causalities of male infertility. To defend itself, the organism comprises many different antioxidants.</p><p><strong>Methods: </strong>We assessed the GPx8 presence in tissues of genital organs from adult rat Sprague-Dawley males by mRNA expression, Western Blot analysis, and immunohistochemistry.</p><p><strong>Results: </strong>The highest mRNA and protein levels were detected in the testis, followed by seminal vesicle. Within testis the enzyme was observed predominantly in the Leydig and Sertoli cells, residual and Hermes bodies. In other organs, such as epididymis, seminal vesicle and prostate gland, the GPx8 was seen in the cytoplasm of epithelial cells. The enzyme was also observed in the muscular layer of hollow organs, in blood plasma and extracellular matrix.</p><p><strong>Conclusions: </strong>The antioxidant enzyme GPx8 was detected in all examined male genital organs. The fact, that the enzyme was released into lumen of genital organs probably means, that GPx8 is also a component of the semen. To our knowledge, this is the first paper describing GPx8 presence in male genital organs of mammals (Fig. 8, Ref. 63).</p>","PeriodicalId":55328,"journal":{"name":"Bratislava Medical Journal-Bratislavske Lekarske Listy","volume":"125 1","pages":"42-49"},"PeriodicalIF":1.5000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bratislava Medical Journal-Bratislavske Lekarske Listy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4149/BLL_2024_008","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0

Abstract

Objectives: The aim of our work was to determine the presence of GPx8, the latest discovered member of glutathione peroxidase family, in rat male genital organs.

Background: The oxidative stress is considered as one of the most important causalities of male infertility. To defend itself, the organism comprises many different antioxidants.

Methods: We assessed the GPx8 presence in tissues of genital organs from adult rat Sprague-Dawley males by mRNA expression, Western Blot analysis, and immunohistochemistry.

Results: The highest mRNA and protein levels were detected in the testis, followed by seminal vesicle. Within testis the enzyme was observed predominantly in the Leydig and Sertoli cells, residual and Hermes bodies. In other organs, such as epididymis, seminal vesicle and prostate gland, the GPx8 was seen in the cytoplasm of epithelial cells. The enzyme was also observed in the muscular layer of hollow organs, in blood plasma and extracellular matrix.

Conclusions: The antioxidant enzyme GPx8 was detected in all examined male genital organs. The fact, that the enzyme was released into lumen of genital organs probably means, that GPx8 is also a component of the semen. To our knowledge, this is the first paper describing GPx8 presence in male genital organs of mammals (Fig. 8, Ref. 63).

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
谷胱甘肽过氧化物酶8 (GPx8)在大鼠雄性生殖器官中的存在。
目的:测定最新发现的谷胱甘肽过氧化物酶家族成员GPx8在大鼠雄性生殖器官中的存在。背景:氧化应激被认为是男性不育最重要的病因之一。为了保护自己,生物体含有许多不同的抗氧化剂。方法:采用mRNA表达、Western Blot、免疫组化等方法检测GPx8在成年雄性sd大鼠生殖器官组织中的表达。结果:mRNA和蛋白水平以睾丸最高,精囊次之。在睾丸内,该酶主要存在于睾丸间质和支持细胞、残体和赫尔姆斯小体中。在其他器官,如附睾、精囊和前列腺,在上皮细胞的细胞质中可见GPx8。在中空器官的肌肉层、血浆和细胞外基质中也观察到该酶。结论:所有男性生殖器官均检测到抗氧化酶GPx8。事实上,这种酶被释放到生殖器官的腔内可能意味着,GPx8也是精液的一种成分。据我们所知,这是第一篇描述GPx8在哺乳动物雄性生殖器官中存在的论文(图8,参考文献63)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
2.60
自引率
0.00%
发文量
185
审稿时长
3-8 weeks
期刊介绍: The international biomedical journal - Bratislava Medical Journal – Bratislavske lekarske listy (Bratisl Lek Listy/Bratisl Med J) publishes peer-reviewed articles on all aspects of biomedical sciences, including experimental investigations with clear clinical relevance, original clinical studies and review articles.
期刊最新文献
Importance of CHB's grey zone: analysis of patients with HBeAg negative chronic hepatitis B virus infection. Prevalence of diastasis m. rectus abdominis and pelvic floor muscle dysfunction in postpartum women. The founding of the UPJS Faculty of Medicine from the memories of the first dean Prof. MUDr. Jan Knazovicky. 75th anniversary of the UPJS Faculty of Medicine in Kosice. The presence of glutathione peroxidase 8 (GPx8) in rat male genital organs. Use of hepatocyte transplantation after extensive liver resections in experiment.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1