Verifying the Equivalence of Hawthorn Leaves Standard Decoction and Formula Granules by LC-MS and Oxidative Stress Test.

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of chromatographic science Pub Date : 2025-02-08 DOI:10.1093/chromsci/bmad089

Ling-Di Wang, Jing-Yun Gao, Li-Ying Duan, Hai-Feng Pan

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Abstract

Objective: To verify the equivalence of hawthorn leaves standard decoction and formula granules.

Methods: In this experiment, liquid chromatograph mass spectrometer (LC-MS) was used to examine the chemical composition of hawthorn leaves standard decoction and formula granules, separately. In addition, oxidative stress test was used to explore the antioxidant capacity of them.

Results: 71 chemical components were identified by LC-MS. Among them, 64 and 56 compounds were identified in the standard decoction and formula granules, respectively. There were a total of 49 common components, with no significant difference in content. Oxidative stress test showed that hawthorn leaves standard decoction and formula granules had no obvious toxicity to human umbilical vein endothelial cells. Compared with the model group, the same dose of hawthorn leaves formula granule and standard decoction could inhibit the secretion of lactate dehydrogenase and malondialdehyde (P < 0.05), and increase the content of superoxide dismutase (P < 0.01), with no statistically significant difference.

Conclusions: There is no significant difference in the main active ingredients between the standard decoction and the formula granules, and the antioxidant activity in vitro is equivalent, providing an important theoretical basis for the further development of hawthorn leaves formula granules.

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用LC-MS和氧化应激试验验证山楂叶标准汤和配方颗粒的等效性。

目的:验证山楂叶标准汤剂与配方颗粒的等效性。方法:采用液相色谱-质谱联用技术，分别对山楂叶标准汤剂和配方颗粒进行化学成分分析。此外，通过氧化应激试验探讨了它们的抗氧化能力。结果:经LC-MS鉴别出71种化学成分。其中，在标准汤剂和配方颗粒中分别鉴定出64种和56种化合物。共有49种成分，在含量上无显著差异。氧化应激试验表明，山楂叶标准汤和配方颗粒对人脐静脉内皮细胞无明显毒性作用。与模型组比较，相同剂量山楂叶配方颗粒与标准汤剂均能抑制小鼠乳酸脱氢酶和丙二醛的分泌(P)。结论:标准汤剂与配方颗粒的主要有效成分无显著差异，体外抗氧化活性相当，为山楂叶配方颗粒的进一步开发提供了重要的理论依据。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.