Regulatory role of ceRNA network in B lymphocytes of patients with immune thrombocytopenia.

IF 3.3 4区医学 Q3 IMMUNOLOGY Autoimmunity Pub Date : 2023-12-01 Epub Date: 2023-12-06 DOI:10.1080/08916934.2023.2281225

Xin He, Nianbin Li, Donglan Liu, Mengtong Zang, Manjun Zhao, Ningyuan Ran, Chunyan Liu, Limin Xing, Huaquan Wang, Ting Wang, Zonghong Shao

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Abstract

Objective: High-throughput sequencing was used to screen expressing differences of miRNA, lncRNA, and mRNA in CD19+ B peripheral blood samples of newly diagnosed immune thrombocytopenia (ITP) patients and healthy controls. The study aimed to explore the regulatory role of ceRNA network in the pathogenesis of dysfunctional CD19 + B lymphocytes of ITP patients.

Methods: CD19+ B lymphocytes were isolated from peripheral blood samples of ITP patients and their healthy counterparts. High-throughput sequencing was used to screen for the expression of miRNA, lncRNA, and mRNA of ITP patients and healthy controls, which were analysed by the ceRNA network. Moreover, qPCR was used to verify the differential expression of miRNA, lncRNA, and mRNA in ITP patients and healthy controls. The correlation between differentially expressed miRNA, lncRNA, mRNA, and B lymphocyte subsets was also analysed.

Results: The CD19+ B lymphocytes of 4 newly diagnosed ITP patients and 4 healthy controls were sequenced and analysed. There were 65 differentially expressed lncRNA and 149 mRNA forming a ceRNA network showed that 12 lncRNA and 136 differentially expressed mRNA were closely associated. Similarly, miR-144-3p, miR-374c-3p, and miR-451a were highly expressed in ITP patients, as confirmed by qPCR, which was consistent with the high-throughput sequence results. LOC102724852 and CCL20 were highly expressed in ITP patients, while LOC105378901, LOC112268311, ALAS2, and TBC1D3F were not as compared to healthy controls, which was consistent with the high-throughput sequence results. In addition, the expression of miR-374c-3p, LOC112268311, LOC105378901, and CXCL3 were correlated with the percentage of B lymphocyte subsets.

Conclusions: The ceRNA network of miRNA, lncRNA, and mRNA in peripheral CD19 + B lymphocytes plays an essential role in the pathogenesis of ITP.

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免疫性血小板减少症患者 B 淋巴细胞中 ceRNA 网络的调控作用

目的：采用高通量测序技术筛选新诊断的免疫性血小板减少症（ITP）患者和健康对照者CD19+B外周血样本中miRNA、lncRNA和mRNA的表达差异。该研究旨在探讨ceRNA网络在ITP患者CD19+B淋巴细胞功能障碍发病机制中的调控作用：方法：从ITP患者及其健康对照者的外周血样本中分离出CD19+ B淋巴细胞。采用高通量测序技术筛选了ITP患者和健康对照组的miRNA、lncRNA和mRNA的表达，并通过ceRNA网络对其进行了分析。此外，研究还利用 qPCR 验证了 miRNA、lncRNA 和 mRNA 在 ITP 患者和健康对照组中的差异表达。研究还分析了差异表达的 miRNA、lncRNA、mRNA 与 B 淋巴细胞亚群之间的相关性：对 4 名新诊断的 ITP 患者和 4 名健康对照者的 CD19+ B 淋巴细胞进行了测序和分析。结果显示，12个lncRNA和136个差异表达的mRNA密切相关。同样，经 qPCR 证实，miR-144-3p、miR-374c-3p 和 miR-451a 在 ITP 患者中高表达，这与高通量序列结果一致。与健康对照组相比，LOC102724852 和 CCL20 在 ITP 患者中高表达，而 LOC105378901、LOC112268311、ALAS2 和 TBC1D3F 则不表达，这与高通量序列结果一致。此外，miR-374c-3p、LOC112268311、LOC105378901和CXCL3的表达与B淋巴细胞亚群的百分比相关：结论：外周 CD19 + B 淋巴细胞中由 miRNA、lncRNA 和 mRNA 组成的 ceRNA 网络在 ITP 的发病机制中起着至关重要的作用。

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来源期刊

Autoimmunity 医学-免疫学

CiteScore

5.70

自引率

8.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Autoimmunity is an international, peer reviewed journal that publishes articles on cell and molecular immunology, immunogenetics, molecular biology and autoimmunity. Current understanding of immunity and autoimmunity is being furthered by the progress in new molecular sciences that has recently been little short of spectacular. In addition to the basic elements and mechanisms of the immune system, Autoimmunity is interested in the cellular and molecular processes associated with systemic lupus erythematosus, rheumatoid arthritis, Sjogren syndrome, type I diabetes, multiple sclerosis and other systemic and organ-specific autoimmune disorders. The journal reflects the immunology areas where scientific progress is most rapid. It is a valuable tool to basic and translational researchers in cell biology, genetics and molecular biology of immunity and autoimmunity.