An immunohistochemical and biomechanical evaluation of the effect of fat graft on tendon healing.

IF 1.6 4区 医学 Journal of Orthopaedic Surgery Pub Date : 2023-09-01 DOI:10.1177/10225536231220839
Ahmet Oztermeli, Mehmet Fatih Okyay
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Abstract

Aim: To investigate the efficacy of fat grafting in primary tendon healing through immunohistochemical and biomechanical examinations.

Material and method: The study material comprised a total of 10 male Sprague-Dawley rats, each approximately 10 weeks old. All 10 rats were operated on bilaterally. The right Achilles tendon in all the animals was defined as the study group. The tendon was cut and then repaired, and then fat graft was applied to the repair area. The left Achilles tendon of all the rats constituted the control group. The tendon was cut and repaired with no further application. After 4 weeks, the rats were euthanised and samples were taken from the tendons for immunohistochemical and biomechanical examinations.

Results: In the biomechanical evaluations, no statistically significant difference was determined between the groups in respect of peak load and stiffness values (p: .068, p: .089, respectively). In the histopathological evaluation, the tenocyte value of the study group was superior to that of the control group (p: .04). No statistically significant differences were determined between the groups in respect of the other histopathological parameters. In the immunohistochemical evaluations, the type I collagen and TGF values of the study group were found to be higher than those of the control group (p: .011, p: .012, respectively).

Conclusion: Compared to stem cell applications, the use of fat grafting is clinically easy to apply, has low costs, and has been shown to contribute to tendon healing at an immunohistochemical level with increased collagen and TGF beta values.

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对脂肪移植对肌腱愈合的影响进行免疫组化和生物力学评估。
目的:通过免疫组化和生物力学检查,研究脂肪移植对原发性肌腱愈合的功效:研究材料包括 10 只雄性 Sprague-Dawley 大鼠,每只约 10 周大。所有 10 只大鼠均接受了双侧手术。所有动物的右跟腱被定义为研究组。切开肌腱后进行修复,然后在修复区域进行脂肪移植。所有大鼠的左跟腱为对照组。对肌腱进行切割和修复,不再应用其他材料。4 周后,大鼠被安乐死,并从肌腱中提取样本进行免疫组化和生物力学检查:在生物力学评估中,各组之间在峰值负荷和硬度值方面没有统计学意义上的差异(P:068,P:089)。在组织病理学评估中,研究组的腱细胞值优于对照组(P:0.04)。研究组与对照组在其他组织病理学参数方面没有明显的统计学差异。在免疫组化评估中,研究组的I型胶原蛋白和TGF值高于对照组(P:0.011,P:0.012):结论:与干细胞应用相比,脂肪移植在临床上易于应用,成本较低,且在免疫组化水平上有助于肌腱愈合,增加胶原蛋白和TGF beta值。
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期刊介绍: Journal of Orthopaedic Surgery is an open access peer-reviewed journal publishing original reviews and research articles on all aspects of orthopaedic surgery. It is the official journal of the Asia Pacific Orthopaedic Association. The journal welcomes and will publish materials of a diverse nature, from basic science research to clinical trials and surgical techniques. The journal encourages contributions from all parts of the world, but special emphasis is given to research of particular relevance to the Asia Pacific region.
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