Imbalance of Follicular Helper and Follicular Regulatory T Cells in Chronic Rhinosinusitis with Nasal Polyps.

Q3 Medicine International Tinnitus Journal Pub Date : 2023-12-04 DOI:10.5935/0946-5448.20230003

Asmaa M Zahran, Omnia El-Badawy, Eman Hosny M Ibrahim, Khaled Saad, Abobakr Abdelmoghny, Amira Elhoufey, Hamad Ghaleb Dailah, Mohamed M Osman

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Abstract

Objective: Data regarding the imbalance in follicular helper T (Tfh) and follicular regulatory T (Tfr) cell responses in patients having chronic rhinosinusitis with nasal polyps (CRSwNP) is so far limited. Thus, we aimed to assess the changes in circulating Tfh and Tfr in CRSwNP patients.

Methods: This case-control study included 21 patients having CRSwNP and 20 age and sex-matched healthy blood donors as a control group. Lund-Mackay staging system was used for radiologic scoring of chronic rhinosinusitis. Two milliliters of peripheral blood samples were collected from all participants into EDTA-containing vacutainer tubes to assess the levels of Tfh and Tfr cells using flow cytometry.

Results: Patients having CRSwNP did not show significant differences in the percentages of CD4+ T cells and total CD4+CXCR5+ T cells from healthy controls. Meanwhile, levels of both activated circulating Tfh and Tfr showed a marked rise in patients than controls. In addition, a positive correlation was observed between the levels of both activated Tfh and Tfr cells.

Conclusion: An imbalance in circulating Tfh/Tfr levels was detected in patients having CRSwNP. A significant rise in the levels of Tfh and Tfr was detected in patients proposing a possible role of this imbalance in disease pathogenesis.

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慢性鼻窦炎伴鼻息肉患者的滤泡辅助性 T 细胞和滤泡调节性 T 细胞失衡。

目的：迄今为止，有关慢性鼻炎伴鼻息肉（CRSwNP）患者的滤泡辅助性T细胞（Tfh）和滤泡调节性T细胞（Tfr）反应失衡的数据还很有限。因此，我们旨在评估 CRSwNP 患者循环 Tfh 和 Tfr 的变化：这项病例对照研究包括 21 名 CRSwNP 患者和 20 名年龄和性别匹配的健康献血者作为对照组。采用 Lund-Mackay 分期系统对慢性鼻炎进行放射学评分。所有参与者的外周血样本均采集于含 EDTA 的真空采血管中，用流式细胞术评估 Tfh 和 Tfr 细胞的水平：结果：CRSwNP 患者的 CD4+ T 细胞百分比和 CD4+CXCR5+ T 细胞总数与健康对照组无明显差异。同时，患者的活化循环 Tfh 和 Tfr 水平均比对照组明显升高。此外，活化的 Tfh 和 Tfr 细胞水平之间呈正相关：结论：CRSwNP 患者的循环 Tfh/Tfr 水平失衡。结论：在 CRSwNP 患者中检测到循环 Tfh/Tfr 水平失衡，患者体内 Tfh 和 Tfr 水平明显升高，表明这种失衡可能与疾病发病机制有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Tinnitus Journal Medicine-Otorhinolaryngology

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： The International Tinnitus Journal is the first peer review journal to provide a forum for exchange of information of on-going basic and clinical science efforts for understanding tinnitus and its application to patient diagnosis and treatment. Subject areas to be covered range from fundamental theory to clinical applications.