COVID-19: detection methods in post-mortem samples.

IF 4.4 Q1 PATHOLOGY PATHOLOGICA Pub Date : 2023-10-01 DOI:10.32074/1591-951X-933
Ilaria Tedesco, Federica Zito Marino, Andrea Ronchi, Amaro Nunes Duarte Neto, Marisa Dolhnikoff, Maurizio Municinò, Carlo Pietro Campobasso, Giuseppe Pannone, Renato Franco
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Abstract

COVID-19 identification is routinely performed on fresh samples, such as nasopharyngeal and oropharyngeal swabs, even if, the detection of the virus in formalin-fixed paraffin-embedded (FFPE) autopsy tissues could help to underlie mechanisms of the pathogenesis that are not well understood.

The gold standard for COVID-19 detection in FFPE samples remains the qRT-PCR as in swab samples, contextually other methods have been developed, including immunohistochemistry (IHC), and in situ hybridization (ISH). In this manuscript, we summarize the main data regarding the methods of COVID-19 detection in pulmonary and extra-pulmonary post-mortem samples, and especially the sensitivity and specificity of these assays will be discussed.

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COVID-19:尸体样本中的检测方法。
尽管在福尔马林固定石蜡包埋(FFPE)尸检组织中检测 COVID-19 有助于揭示尚不十分清楚的发病机制,但对新鲜样本(如鼻咽和口咽拭子)进行 COVID-19 鉴定仍是常规做法。在 FFPE 样本中检测 COVID-19 的金标准仍然是 qRT-PCR,而在拭子样本中则采用其他方法,包括免疫组化(IHC)和原位杂交(ISH)。在本手稿中,我们将总结有关肺部和肺外尸检样本中 COVID-19 检测方法的主要数据,并特别讨论这些检测方法的灵敏度和特异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
PATHOLOGICA
PATHOLOGICA PATHOLOGY-
CiteScore
5.90
自引率
5.70%
发文量
108
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