Intra-accumbal orexinergic system contributes to the stress-induced antinociceptive behaviors in the animal model of acute pain in rats.

IF 1.6 4区 心理学 Q3 BEHAVIORAL SCIENCES Behavioural Pharmacology Pub Date : 2024-04-01 Epub Date: 2024-02-23 DOI:10.1097/FBP.0000000000000763
Mohammad Nikoohemmat, Danial Farmani, Seyed Mohammadmisagh Moteshakereh, Sakineh Salehi, Laleh Rezaee, Abbas Haghparast
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Abstract

Stress and pain are interleaved at numerous levels - influencing each other. Stress can increase the nociception threshold in animals, long-known as stress-induced analgesia (SIA). Orexin is known as a neuropeptide that modulates pain. The effect of stress on the mesolimbic system in the modulation of pain is known. The role of the intra-accumbal orexin receptors in the modulation of acute pain by forced swim stress (FSS) is unclear. In this study, 117 adult male albino Wistar rats (270-300 g) were used. The animals were unilaterally implanted with cannulae above the NAc. The antagonist of the orexin-1 receptor (OX1r), SB334867, and antagonist of the orexin-2 receptor (OX2r), TCS OX2 29, were microinjected into the NAc in different doses (1, 3, 10, and 30 nmol/0.5 µl DMSO) before exposure to FSS for a 6-min period. The tail-flick test was carried out as an assay nociception of acute pain, and the nociceptive threshold [tail-flick latency (TFL)] was measured for 60-minute. The findings demonstrated that exposure to acute stress could remarkably increase the TFLs and antinociceptive responses. Moreover, intra-accumbal microinjection of SB334867 or TCS OX2 29 blocked the antinociceptive effect of stress in the tail-flick test. The contribution of orexin receptors was almost equally modulating SIA. The present study's findings suggest that OX1r and OX2r within the NAc modulate stress-induced antinociceptive responses. The intra-accumbal microinjection of orexin receptors antagonists declares inducing antinociceptive responses by FSS in acute pain. Proposedly, intra-accumbla orexinergic receptors have a role in the development of SIA.

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在急性疼痛动物模型中,伏核内食欲能系统参与应激诱导的抗伤害感受行为。
压力和痛苦在许多层面上交织在一起,相互影响。应激可以增加动物的痛觉阈值,长期以来被称为应激性镇痛(SIA)。食欲素是一种调节疼痛的神经肽。在疼痛调节中,压力对中脑边缘系统的影响是已知的。伏核内食欲素受体在强迫游泳应激(FSS)引起的急性疼痛调节中的作用尚不清楚。本研究选用成年雄性白化Wistar大鼠117只(270 ~ 300 g)。动物在NAc上方单侧植入套管。将orexin-1受体拮抗剂(OX1r) SB334867和orexin-2受体拮抗剂(OX2r) TCS ox229以不同剂量(1、3、10和30 nmol/0.5µl DMSO)微量注射到NAc中,然后暴露于FSS 6分钟。甩尾实验作为急性疼痛的伤害感受测试,并测量60分钟的伤害感受阈值[甩尾潜伏期(tail-flick latency, TFL)]。结果表明,暴露于急性应激可以显著增加TFLs和抗伤害反应。此外,在甩尾实验中,伏隔区内微量注射SB334867或TCS OX2 29可阻断应激的抗伤害感受作用。食欲素受体对SIA的调节作用几乎相同。本研究结果表明,NAc中的OX1r和OX2r调节应激诱导的抗伤害反应。伏隔区微注射食欲素受体拮抗剂可诱导FSS对急性疼痛的抗感觉反应。因此,伏隔核内食欲能受体在SIA的发展中起作用。
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来源期刊
Behavioural Pharmacology
Behavioural Pharmacology 医学-行为科学
CiteScore
3.40
自引率
0.00%
发文量
84
审稿时长
6-12 weeks
期刊介绍: Behavioural Pharmacology accepts original full and short research reports in diverse areas ranging from ethopharmacology to the pharmacology of schedule-controlled operant behaviour, provided that their primary focus is behavioural. Suitable topics include drug, chemical and hormonal effects on behaviour, the neurochemical mechanisms under-lying behaviour, and behavioural methods for the study of drug action. Both animal and human studies are welcome; however, studies reporting neurochemical data should have a predominantly behavioural focus, and human studies should not consist exclusively of clinical trials or case reports. Preference is given to studies that demonstrate and develop the potential of behavioural methods, and to papers reporting findings of direct relevance to clinical problems. Papers making a significant theoretical contribution are particularly welcome and, where possible and merited, space is made available for authors to explore fully the theoretical implications of their findings. Reviews of an area of the literature or at an appropriate stage in the development of an author’s own work are welcome. Commentaries in areas of current interest are also considered for publication, as are Reviews and Commentaries in areas outside behavioural pharmacology, but of importance and interest to behavioural pharmacologists. Behavioural Pharmacology publishes frequent Special Issues on current hot topics. The editors welcome correspondence about whether a paper in preparation might be suitable for inclusion in a Special Issue.
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