Puerarin protects renal ischemia-reperfusion injury in rats through NLRP3/Caspase-1/GSDMD pathway.

Acta cirurgica brasileira Pub Date : 2023-12-01 eCollection Date: 2023-01-01 DOI:10.1590/acb387323

Kangyu Wang, Zhao Tang, Shuai Liu, Yan Liu, Huiqing Zhang, Haocheng Zhan

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Abstract

Purpose: To observe the effect of puerarin on renal ischemia-reperfusion (I/R) injury in rats, and to explore its mechanism based on NLRP3/Caspase-1/GSDMD pathway.

Methods: Twenty-one Sprague-Dawley rats were divided into three groups: sham-operated group (sham), model group (RIRI), and puerarin treatment group (RIRI + Pue). The model of acute renal I/R injury was established by cutting the right kidney and clamping the left renal pedicle for 45 min.

Results: Renal function parameters were statistically significant in group comparisons. The renal tissue structure of rats in sham group was basically normal. Pathological changes were observed in the RIRI group. The renal pathological damage score and apoptosis rate in the RIRI group were higher than those in the sham group, and significantly lower in the RIRI + Pue group than in the RIRI group. Indicators of oxidative stress-superoxide dismutase, malondialdehyde, and glutathione peroxidase-were statistically significant in group comparisons. Compared with the sham group, the relative expressions of NLRP3, Caspase-1 and GSDMD proteins in the RIRI group were increased. Compared with the RIRI group, the RIRI + Pue group had significant reductions.

Conclusions: Puerarin can inhibit the activation of NLRP3/Caspase-1/GSDMD pathway, inhibit inflammatory response and pyroptosis, and enhance the antioxidant capacity of kidney, thereby protecting renal I/R injury in rats.

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葛根素通过NLRP3/Caspase-1/GSDMD通路保护大鼠肾缺血再灌注损伤。

目的:观察葛根素对大鼠肾缺血再灌注(I/R)损伤的影响，并基于NLRP3/Caspase-1/GSDMD通路探讨其作用机制。方法:21只sd大鼠分为3组:假手术组(sham)、模型组(RIRI)和葛根素治疗组(RIRI + Pue)。采用右肾切开、左肾蒂夹持45 min的方法，建立急性肾I/R损伤模型。结果:各组比较肾功能指标差异均有统计学意义。假手术组大鼠肾脏组织结构基本正常。RIRI组观察病理改变。RIRI组肾脏病理损伤评分及凋亡率均高于sham组，RIRI + Pue组明显低于RIRI组。氧化应激指标超氧化物歧化酶、丙二醛、谷胱甘肽过氧化物酶组间比较均有统计学意义。与sham组比较，RIRI组NLRP3、Caspase-1、GSDMD蛋白的相对表达量升高。与RIRI组相比，RIRI + Pue组有显著降低。结论:葛根素可抑制NLRP3/Caspase-1/GSDMD通路的激活，抑制炎症反应和焦亡，增强肾脏的抗氧化能力，从而保护大鼠肾I/R损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta cirurgica brasileira

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