Chemical Composition Analysis and Multi-index Component Content Determination of Compounds in Goupi Plaster Based on UPLC–Q-Exactive-MS and UPLC–MS/MS

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Chromatographia Pub Date : 2023-11-20 DOI:10.1007/s10337-023-04296-7

Yunfeng Xue, Tong Guan, Jia Liu, Jiajing Wang, Zhixin Yang, Feng Guan, Weinan Li, Yanhong Wang

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Abstract

Objective

This study sought to evaluate and validate a method for chemical composition analysis and content determination of Goupi plaster components, a conventional prescription preparation of traditional Chinese medicine. This is geared toward providing a basis for quality control research and future development of Goupi plaster.

Methods

UPLC–Q-Exactive-MS was used to qualitatively analyze the chemical components of Goupi plaster from different manufacturers in positive and negative ion modes. UPLC–MS/MS method was used to establish the determination methods for the detection of sinomenine, osthole, and cinnamaldehyde in Goupi plaster from different manufacturers.

Results

A total of 291 chemical components were identified in Goupi plaster from four manufacturers, including 97 chemical components with known source and pharmacological activity. Further, we determined the contents of sinomenine, osthole, and cinnamaldehyde.

Conclusion

In summary, the UPLC–Q-Exactive-MS method was used to analyze the chemical components of Goupi plaster from different manufacturers. We established the UPLC–MS/MS method to determine the contents of sinomenine, osthole, and cinnamaldehyde in Goupi plaster from different manufacturers. The findings indicated that the method was comprehensive, rapid, and accurate, preliminarily revealing the material basis of Goupi plaster and providing a reference for follow-up development of Goupi plaster.

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基于uplc - q - hplc -MS和UPLC-MS /MS的狗皮膏化学成分分析及多指标成分含量测定

目的评价和验证中药常规方剂勾皮膏的化学成分分析及含量测定方法。为狗皮膏药的质量控制研究和今后的开发提供依据。方法采用suplc - q - exactive - ms对不同厂家勾皮膏的化学成分进行正离子和负离子模式定性分析。采用UPLC-MS /MS法建立了不同厂家勾皮膏中青藤碱、蛇床子碱和肉桂醛的检测方法。结果4家生产企业的狗皮膏共检出化学成分291种，其中来源已知、药理活性已知的化学成分97种。进一步测定了青藤碱、蛇床子素和肉桂醛的含量。综上所述，采用UPLC-Q-Exactive-MS方法可对不同生产厂家的勾皮膏进行化学成分分析。建立了hplc - MS/MS法测定不同厂家勾皮膏中青藤碱、蛇床子碱和肉桂醛的含量。结果表明，该方法全面、快速、准确，初步揭示了勾皮膏的物质基础，为勾皮膏的后续开发提供参考。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.