Physiology and transcriptomics reveal that hybridization improves the tolerance of poplar photosynthetic function to salt stress

IF 2.1 3区农林科学 Q2 FORESTRY Trees Pub Date : 2023-12-04 DOI:10.1007/s00468-023-02468-4

Ding Changjun, Wang Yue, Zhang Weixi, Wang Jiechen, Song Jiaqi, Cui Congcong, Ji Guangxin, Ding Mi, Su Xiaohua, Zhang Huihui

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Key message

Significant improvement in salt tolerance of poplar hybrids with different salt 2 tolerant varieties.

Abstract

Salt stress is a global environmental factor that limits plant growth and productivity. Poplar has the characteristics of fast growth and fine texture, and the adaptability of hybrid plants to environmental stress is better than that of their parents. In this study, Populus simonii ‘CAFDF’ (♀), P. nigra ‘CAFDM’ (♂), P. simonii × P. nigra CV. ‘CAFDS2’ (DS2), and Populus simonii × P. nigra CV. ‘CAFDS3’ (DS3) were used to reveal the response and adaptation mechanism of the photosynthetic function of hybrid poplar leaves to salt stress by physiological combined with transcriptome technology. The results showed that NaCl stress significantly reduced the chlorophyll content of the leaves of both parents, but the reduction of chlorophyll in hybrid offspring under salt stress was lower than that in parents. A Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed genes (DEGs) in both hybrid plants and biparental progenies under NaCl stress was enriched for photosynthesis-antenna proteins, photosynthesis, and carbon fixation in photosynthetic organisms. Under salt stress treatment, the ability of leaves in DS2 and DS3 plants to capture solar energy and transfer their excitement energy to the reaction center were higher than that of their parental plants. NaCl stress caused damage to photosystem II (PSII) and photosystem I (PSI) in poplar leaves, and their activities decreased. The hybrid offspring improved the integrity of PSII receptor side electron transfer and donor side oxygen-evolving complex (OEC) compared with the two parents. The expression of other coding genes of the PSII reaction center, as well as coding genes for PSI reaction center and ATP synthetase in the hybrid offspring under NaCl stress are higher than that of the parents, which reduces damage to the electron transfer chain and the energy supply required for maintaining plant growth and development. The cyclic electron flow dependent on PGR5 and NDH pathways (PGR5-CEF and NDH-CEF) of hybrids and female parents were significantly higher than those of male parents. Hybrid progeny and the maternal parent alleviated the damage of salt stress on PSII and PSI by promoting CEF. The hybrid plants protect the photosynthetic apparatus by regulating energy dissipation, while the PSII of the parents plants was damaged due to serious photooxidation. Under salt stress, the net photosynthetic rate (P_n) in hybrid plants increased, and the non-stomatal factors, which was also confirmed by the Calvin cycle.

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生理和转录组学研究表明，杂交提高了杨树光合功能对盐胁迫的耐受性

不同耐盐品种杂交杨树耐盐性显著提高。摘要盐胁迫是制约植物生长和生产力的全球性环境因子。杨树具有生长快、质地细腻的特点，杂交植株对环境胁迫的适应性优于亲本。在本研究中，西孟杨‘CAFDF’(♀)、黑胡杨‘CAFDM’(♂)、西孟杨×黑胡杨CV;‘CAFDS2’(DS2)和胡杨(Populus simonii × P. nigra) CV。利用生理结合转录组技术，研究杂交杨树叶片光合功能对盐胁迫的响应及适应机制。结果表明，NaCl胁迫显著降低了亲本叶片叶绿素含量，但杂交后代在盐胁迫下叶绿素含量的降低幅度低于亲本。《京都基因与基因组百科全书》(KEGG)富集分析了NaCl胁迫下杂交植物和双亲本后代的差异表达基因(DEGs)，富集了光合生物的光合天线蛋白、光合作用和固碳作用。盐胁迫下，DS2和DS3植株叶片捕捉太阳能并将兴奋能传递到反应中心的能力高于亲本植株。NaCl胁迫导致杨树叶片光系统II (PSII)和光系统I (PSI)活性降低。与两个亲本相比，杂交后代的PSII受体侧电子转移和供体侧氧进化复合物(OEC)的完整性有所提高。NaCl胁迫下杂交种其他PSII反应中心编码基因、PSI反应中心编码基因和ATP合成酶编码基因的表达量均高于亲本，减少了对电子传递链的破坏，减少了维持植物生长发育所需的能量供应。杂交种和母本依赖于PGR5和NDH通路(PGR5- cef和NDH- cef)的循环电子流显著高于父本。杂交后代和母本通过促进CEF减轻盐胁迫对PSII和PSI的伤害。杂种植物通过调节能量耗散来保护光合机构，而亲本植物的PSII则因严重的光氧化而受损。在盐胁迫下，杂交植株的净光合速率(Pn)和非气孔因子均有所增加，这一点也得到了卡尔文循环的证实。

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来源期刊

Trees 农林科学-林学

CiteScore

4.50

自引率

4.30%

发文量

113

审稿时长

3.8 months

期刊介绍： Trees - Structure and Function publishes original articles on the physiology, biochemistry, functional anatomy, structure and ecology of trees and other woody plants. Also presented are articles concerned with pathology and technological problems, when they contribute to the basic understanding of structure and function of trees. In addition to original articles and short communications, the journal publishes reviews on selected topics concerning the structure and function of trees.